Transcription of SARS-CoV-2 sgRNAs

Stable Identifier
R-HSA-9694786
Type
Pathway
Species
Homo sapiens
Related Species
Severe acute respiratory syndrome coronavirus 2
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This COVID‑19 pathway has been created by a combination of computational inference from SARS-CoV-1 data (https://reactome.org/documentation/inferred-events) and manual curation, as described in the summation for the overall SARS-CoV-2 infection pathway. Steps of SARS‑CoV‑2 transcription that have been studied directly include binding of the replication‑transcription complex (RTC) to the RNA template and the polymerase activity of nsp12 (Hillen et al. 2020, Wang et al. 2020, Yin et al. 2020), helicase activity of nsp13 (Chen et al. 2020, Ji et al. 2020, Shu et al. 2020), capping activity of nsp16 (Viswanathan et al. 2020), and polyadenylation of SARS‑CoV‑2 transcripts (Kim et al. 2020, Ravindra et al. 2020). Remaining steps have been inferred from previous studies in SARS‑CoV‑1 and related coronaviruses.

SARS-CoV-1 encodes eight subgenomic RNAs, mRNA2 to mRNA9. mRNA1 corresponds to the genomic RNA. The 5' and 3' ends of subgenomic RNAs are identical, in accordance with the template switch model of coronavirus RNA transcription (Snijder et al. 2003, Thiel et al. 2003, Yount et al. 2003). Genomic positive strand RNA is first transcribed into negative sense (minus strand) subgenomic mRNAs by template switching. Negative sense mRNAs subsequently serve as templates for the synthesis of positive strand subgenomic mRNAs. As shown in murine hepatitis virus (MHV), which is closely related to SARS-CoV-1, negative-sense viral RNAs are present in much smaller amounts than positive-sense RNAs (Irigoyen et al. 2016). Of the eight subgenomic mRNAs of SARS-CoV-1, mRNA2 encodes the S protein, mRNA3 is bicistronic and encodes proteins 3a and 3b, mRNA4 encodes the E protein, mRNA5 encodes the M protein, mRNA6 encodes protein 6, and bicistronic mRNA7, mRNA8 and mRNA9 encode proteins 7a and 7b (mRNA7), 8a and 8b (mRNA8), and 9a and N (mRNA9), respectively (Snijder et al. 2003, Thiel et al. 2003, Yount et al. 2003). The template switch model of coronavirus involves discontinuous transcription of subgenomic RNA, with the leader body joining occurring during the synthesis of minus strand RNAs. Each subgenomic RNA contains a leader transcription regulatory sequence (leader TRS) that is identical to the leader of the genome, appended via polymerase “jumping” during negative strand synthesis to the body transcription regulatory sequence (body TRS), a short, AU-rich motif of about 10 nucleotides found upstream of each ORF that is destined to become 5' proximal in one of the subgenomic mRNAs. The 3' and 5'UTRs may interact through RNA–RNA and/or RNA–protein plus protein–protein interactions to promote circularization of the coronavirus genome, placing the elongating minus strand in a favorable topology for leader-body joining. The host protein PABP was found to bind to the coronavirus 3' poly(A) tail and to interact with the host protein eIF-4G, a component of the three-subunit complex that binds to mRNA cap structures, which may promote the circularization of the coronavirus genome. Two viral proteins that bind to the coronavirus 5'UTR, the N protein and nsp1, may play a role in template switching. The poly(A) tail is necessary for the initiation of minus-strand RNA synthesis at the 3' end of genomic RNA. Elongation of nascent minus strand RNA continues until the first functional body TRS motif is encountered. A fixed proportion of replication-transcription complexes (RTCs) will either disregard the TRS motif and continue to elongate the nascent strand or stop synthesis of the nascent minus strand and relocate to the leader TRS, extending the minus strand by copying the 5' end of the genome. The completed minus-strand RNAs then serve as templates for positive strand mRNA synthesis (reviewed by Sawicki et al. 2007, Yang and Leibowitz 2015).

Literature References
PubMed ID Title Journal Year
32511382 Single-cell longitudinal analysis of SARS-CoV-2 infection in human bronchial epithelial cells

Ravindra, NG, Alfajaro, MM, Gasque, V, Wei, J, Filler, RB, Huston, NC, Wan, H, Szigeti-Buck, K, Wang, B, Montgomery, RR, Eisenbarth, SC, Williams, A, Pyle, AM, Iwasaki, A, Horvath, TL, Foxman, EF, van Dijk, D, Wilen, CB

bioRxiv 2020
32484220 Discovery of G-quadruplex-forming sequences in SARS-CoV-2

Ji, D, Juhas, M, Tsang, CM, Kwok, CK, Li, Y, Zhang, Y

Brief. Bioinformatics 2020
12917450 Mechanisms and enzymes involved in SARS coronavirus genome expression

Thiel, V, Ivanov, KA, Putics, Á, Hertzig, T, Schelle, B, Bayer, S, Weißbrich, B, Snijder, EJ, Rabenau, H, Doerr, HW, Gorbalenya, AE, Ziebuhr, J

J. Gen. Virol. 2003
32783916 Structural Basis for Helicase-Polymerase Coupling in the SARS-CoV-2 Replication-Transcription Complex

Chen, J, Malone, B, Llewellyn, E, Grasso, M, Shelton, PMM, Olinares, PDB, Maruthi, K, Eng, ET, Vatandaslar, H, Chait, BT, Kapoor, TM, Darst, SA, Campbell, EA

Cell 2020
12927536 Unique and conserved features of genome and proteome of SARS-coronavirus, an early split-off from the coronavirus group 2 lineage

Snijder, EJ, Bredenbeek, PJ, Dobbe, JC, Thiel, V, Ziebuhr, J, Poon, LL, Guan, Y, Rozanov, M, Spaan, WJ, Gorbalenya, AE

J. Mol. Biol. 2003
32358203 Structural basis for inhibition of the RNA-dependent RNA polymerase from SARS-CoV-2 by remdesivir

Yin, W, Mao, C, Luan, X, Shen, DD, Shen, Q, Su, H, Wang, X, Zhou, F, Zhao, W, Gao, M, Chang, S, Xie, YC, Tian, G, Jiang, HW, Tao, SC, Shen, J, Jiang, Y, Jiang, H, Xu, Y, Zhang, S, Zhang, Y, Xu, HE

Science 2020
25736566 The structure and functions of coronavirus genomic 3' and 5' ends

Yang, D, Leibowitz, JL

Virus Res. 2015
14569023 Reverse genetics with a full-length infectious cDNA of severe acute respiratory syndrome coronavirus

Yount, B, Curtis, KM, Fritz, EA, Hensley, LE, Jahrling, PB, Prentice, E, Denison, MR, Geisbert, TW, Baric, RS

Proc. Natl. Acad. Sci. U.S.A. 2003
32330414 The Architecture of SARS-CoV-2 Transcriptome

Kim, D, Lee, JY, Yang, JS, Kim, JW, Kim, VN, Chang, H

Cell 2020
32500504 SARS-Coronavirus-2 Nsp13 Possesses NTPase and RNA Helicase Activities That Can Be Inhibited by Bismuth Salts

Shu, T, Huang, M, Wu, D, Ren, Y, Zhang, X, Han, Y, Mu, J, Wang, R, Qiu, Y, Zhang, DY, Zhou, X

Virol Sin 2020
16928755 A contemporary view of coronavirus transcription

Sawicki, SG, Sawicki, DL, Siddell, SG

J. Virol. 2007
32709886 Structural basis of RNA cap modification by SARS-CoV-2

Viswanathan, T, Arya, S, Chan, SH, Qi, S, Dai, N, Misra, A, Park, JG, Oladunni, F, Kovalskyy, D, Hromas, RA, Martínez-Sobrido, L, Gupta, YK

Nat Commun 2020
32438371 Structure of replicating SARS-CoV-2 polymerase

Hillen, HS, Kokic, G, Farnung, L, Dienemann, C, Tegunov, D, Cramer, P

Nature 2020
32526208 Structural Basis for RNA Replication by the SARS-CoV-2 Polymerase

Wang, Q, Wu, J, Wang, H, Gao, Y, Liu, Q, Mu, A, Ji, W, Yan, L, Zhu, Y, Zhu, C, Fang, X, Yang, X, Huang, Y, Gao, H, Liu, F, Ge, J, Sun, Q, Yang, X, Xu, W, Liu, Z, Yang, H, Lou, Z, Jiang, B, Guddat, LW, Gong, P, Rao, Z

Cell 2020
Participants
Participates
Disease
Name Identifier Synonyms
COVID-19 DOID:0080600 2019 Novel Coronavirus (2019-nCoV), Wuhan seafood market pneumonia virus infection, 2019-nCoV infection, Wuhan coronavirus infection
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Reviewed
Created
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