nsp12 transfers guanylyl onto SARS-CoV-2 plus strand subgenomic RNAs

Stable Identifier
R-HSA-9815529
Type
Reaction [transition]
Species
Homo sapiens
Related Species
Severe acute respiratory syndrome coronavirus 2
Compartment
cytosol
ReviewStatus
5/5
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nsp12 transfers guanylyl onto SARS-CoV-2 plus strand subgenomic RNAs
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Capping of viral mRNA starts with the transfer of a guanylyl residue onto the RNA`s phosphate moiety, creating the cap core structure (GpppA, also cap(-1)). SARS-CoV-2 Nsp12 has been shown to have this guanylyltransferase (GTase) activity, carrying out the addition of a GTP nucleotide to the 5' end of viral RNA, which can be inhibited by remdesivir triphosphate, the active form of the antiviral drug remdesivir (Yan et al, 2021; Walker et al, 2021).
Literature References
PubMed ID Title Journal Year
33232691 Cryo-EM Structure of an Extended SARS-CoV-2 Replication and Transcription Complex Reveals an Intermediate State in Cap Synthesis

Wang, Q, Lou, Z, Rao, Z, Huang, Y, Gao, Y, Li, M, Li, Y, Yan, L, Liu, Z, Gao, S, Zhang, Y, Ge, J, Chen, Y, Zheng, L, Wang, H, Guddat, LW, Yang, Y, Wang, T

Cell 2021
34850141 The SARS-CoV-2 RNA polymerase is a viral RNA capping enzyme

Keown, JR, Grimes, JM, Walker, AP, Fan, H, Fodor, E, Knight, ML

Nucleic Acids Res 2021
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Catalyst Activity

mRNA guanylyltransferase activity of pp1ab-nsp12 [cytosol]

Physical Entity
pp1ab-nsp12 [cytosol] (Severe acute respiratory syndrome coronavirus 2)
Activity
mRNA guanylyltransferase activity (GO:0004484)
Disease
Name Identifier Synonyms
COVID-19 DOID:0080600 2019 Novel Coronavirus (2019-nCoV), Wuhan seafood market pneumonia virus infection, 2019-nCoV infection, Wuhan coronavirus infection
Authored
Stephan, R  (2022-08-17)
Reviewed
Gillespie, ME  (2022-05-10)
Created
Stephan, R  (2022-08-18)
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