Reactome | Substrate translocates into late endosomal lumen

Substrate translocates into late endosomal lumen

Stable Identifier

R-HSA-9631065

Type

Reaction [transition]

Species

Homo sapiens

Compartment

late endosome membrane, late endosome lumen, cytosol

ReviewStatus

5/5

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Substrate translocates into late endosomal lumen

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In addition to bulk protein degradation, Microautophagy (MI) can also target KFERQ motif protein substrates similar to Chaperone Mediated Autophagy (CMA). However, MI is distinct from CMA in that it occurs during late endosomes/multivesicular bodies formation. Upon binding with Hspa8, substrates are transported from the cytosol to late endosomes. HSPA8 binds with the phospholipids on the late endosomal membrane. Subsequently, the substrate is transported into the lumen via endosomal sorting complexes required for transport (ESCRTI and ESCRTIII systems) (Sahu R et al. 2011). This event is represented as a black box since the precise molecular mechanism of the substrate transport into the endosomal lumen is unclear. Experiments confirming this event were performed in mouse.

Literature References

PubMed ID	Title	Journal	Year
21238931	Microautophagy of cytosolic proteins by late endosomes Sahu, R, Kaushik, S, Clement, CC, Cannizzo, ES, Scharf, B, Follenzi, A, Potolicchio, I, Nieves, E, Cuervo, AM, Santambrogio, L	Dev. Cell	2011