Reactome | RAC3 GAPs stimulate RAC3 GTPase activity

RAC3 GAPs stimulate RAC3 GTPase activity

Stable Identifier

R-HSA-9018806

Type

Reaction [transition]

Species

Homo sapiens

Compartment

plasma membrane, cytosol

ReviewStatus

5/5

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RAC3 GAPs stimulate RAC3 GTPase activity

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The following GTPase activating proteins (GAPs) were shown to bind RAC3 and stimulate its GTPase activity, resulting in GTP to GDP hydrolysis and conversion of the active RAC3:GTP complex into the inactive RAC3:GDP complex (the high throughput screen by Bagci et al. 2020, cited as supporting evidence, examined binding of GAPs to constitutively active RAC3 mutant without looking at activation of the RAC3 GTPase activity):
ARHGAP1 (Amin et al. 2016; supported by Bagci et al. 2020)
ARHGAP6 (Li et al. 2016)
ARHGAP15 (Zamboni et al. 2016)
ARHGAP26 (Amin et al. 2016)
ARHGAP35 (Amin et al. 2016; supported by Bagci et al. 2020)
RACGAP1 (Amin et al. 2016; supported by Bagci et al. 2020)

The following GAPs, annotated as candidate RAC3 GAPs, were shown to bind RAC3 and stimulate its GTPase activity in some but not all studies or were shown to bind to the constitutively active RAC3 mutant without testing for activation of RAC3 GTPase activity, as indicated:
ABR (Amin et al. 2016: RAC3 directed GAP activity; Bagci et al. 2020: no binding to active RAC3)
ARAP2 (Bagci et al. 2020: binding to active RAC3)
ARAP3 (Bagci et al. 2020: binding to active RAC3)
ARHGAP5 (Bagci et al. 2020: binding to active RAC3)
ARHGAP17 (Amin et al. 2016: RAC3 directed GAP activity; Bagci et al. 2020: no binding to active RAC3)
ARHGAP21 (Bagci et al. 2020: binding to active RAC3)
ARHGAP32 (Bagci et al. 2020: binding to active RAC3)
ARHGAP39 (Bagci et al. 2020: binding to active RAC3)
ARHGAP42 (Bagci et al. 2020: binding to active RAC3)
BCR (Haataja et al. 1997: RAC3 directed GAP activity; Bagci et al. 2020: no binding to active RAC3)
DEPDC1B (Bagci et al. 2020: binding to active RAC3)
OCRL (Bagci et al. 2020: binding to active RAC3; Erdmann et al. 2007; Lichter Konecki et al. 2006: no RAC3 directed GAP activity:)
OPHN1 (Amin et al. 2016: RAC3 directed GAP activity; Bagci et al. 2020: no binding to active RAC3)
PIK3R1 (Bagci et al. 2020: binding to active RAC3)
PIK3R2 (Bagci et al. 2020: binding to active RAC3)
SRGAP2 (Bagci et al. 2020: binding to active RAC3)
SYDE1 (Bagci et al. 2020: binding to active RAC3)

The following GAPs have been shown to not act on RAC3 or were shown to not bind to the constitutively active RAC3 mutant by Bagci et al. 2020:
ARHGAP12 (Bagci et al. 2020)
ARHGAP29 (Bagci et al. 2020)
ARHGAP31 (Bagci et al. 2020)
DLC1 (Amin et al. 2016)
MYO9A (Bagci et al. 2020)
MYO9B (Bagci et al. 2020)
STARD13 (Amin et al. 2016)
STARD8 (Amin et al. 2016)

Literature References

PubMed ID	Title	Journal	Year
26628301	Inhibitory effects of Arhgap6 on cervical carcinoma cells Liu, Y, Li, J, Yin, Y	Tumour Biol.	2016
27481945	Deciphering the Molecular and Functional Basis of RHOGAP Family Proteins: A SYSTEMATIC APPROACH TOWARD SELECTIVE INACTIVATION OF RHO FAMILY PROTEINS Ahmadian, MR, Somlyo, AV, Amin, E, Koessmeier, KT, Jaiswal, M, Dvorsky, R, Reis, K, Derewenda, U, Nouri, K, Aspenström, P	J. Biol. Chem.	2016
17765681	A role of the Lowe syndrome protein OCRL in early steps of the endocytic pathway Paradise, S, Zoncu, R, Lee, S, Mao, Y, Biemesderfer, D, McCrea, HJ, Toomre, D, De Camilli, P, Erdmann, KS, Modregger, J	Dev. Cell	2007
16777452	The effect of missense mutations in the RhoGAP-homology domain on ocrl1 function Nussbaum, RL, Lichter-Konecki, U, Suchy, SF, Farber, LW, Cronin, JS	Mol. Genet. Metab.	2006
31871319	Mapping the proximity interaction network of the Rho-family GTPases reveals signalling pathways and regulatory mechanisms Tran, V, Gingras, AC, Elkholi, IE, Robert, A, Boulais, J, Faubert, D, Dubé, N, Hipfner, DR, Cote, JF, Lin, ZY, Bagci, H, Sriskandarajah, N, Thibault, MP	Nat. Cell Biol.	2020
9252344	Characterization of RAC3, a novel member of the Rho family Heisterkamp, N, Haataja, L, Groffen, J	J. Biol. Chem.	1997
27713499	Disruption of ArhGAP15 results in hyperactive Rac1, affects the architecture and function of hippocampal inhibitory neurons and causes cognitive deficits Ciraolo, E, Zamboni, V, El-Assawi, N, Sarò, G, Hirsch, E, Mauro, A, Umbach, A, Priano, L, Ferri, N, D'Adamo, P, Germena, G, Ghigo, A, Passafaro, M, Carabelli, V, Merlo, GR, Bianchi, V, Armentano, M, Gavello, D, de Curtis, I, Valnegri, P	Sci Rep	2016