RAC3 GAPs stimulate RAC3 GTPase activity

Stable Identifier
R-HSA-9018806
Type
Reaction [transition]
Species
Homo sapiens
Compartment
Locations in the PathwayBrowser
General
SVG |   | PPTX  | SBGN
Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

The following GTPase activating proteins (GAPs) were shown to bind RAC3 and stimulate its GTPase activity, resulting in GTP to GDP hydrolysis and conversion of the active RAC3:GTP complex into the inactive RAC3:GDP complex (the high throughput screen by Bagci et al. 2020, cited as supporting evidence, examined binding of GAPs to constitutively active RAC3 mutant without looking at activation of the RAC3 GTPase activity):
ARHGAP1 (Amin et al. 2016; supported by Bagci et al. 2020)
ARHGAP6 (Li et al. 2016)
ARHGAP15 (Zamboni et al. 2016)
ARHGAP26 (Amin et al. 2016)
ARHGAP35 (Amin et al. 2016; supported by Bagci et al. 2020)
RACGAP1 (Amin et al. 2016; supported by Bagci et al. 2020)

The following GAPs, annotated as candidate RAC3 GAPs, were shown to bind RAC3 and stimulate its GTPase activity in some but not all studies or were shown to bind to the constitutively active RAC3 mutant without testing for activation of RAC3 GTPase activity, as indicated:
ABR (Amin et al. 2016: RAC3 directed GAP activity; Bagci et al. 2020: no binding to active RAC3)
ARAP2 (Bagci et al. 2020: binding to active RAC3)
ARAP3 (Bagci et al. 2020: binding to active RAC3)
ARHGAP5 (Bagci et al. 2020: binding to active RAC3)
ARHGAP17 (Amin et al. 2016: RAC3 directed GAP activity; Bagci et al. 2020: no binding to active RAC3)
ARHGAP21 (Bagci et al. 2020: binding to active RAC3)
ARHGAP32 (Bagci et al. 2020: binding to active RAC3)
ARHGAP39 (Bagci et al. 2020: binding to active RAC3)
ARHGAP42 (Bagci et al. 2020: binding to active RAC3)
BCR (Haataja et al. 1997: RAC3 directed GAP activity; Bagci et al. 2020: no binding to active RAC3)
DEPDC1B (Bagci et al. 2020: binding to active RAC3)
OCRL (Bagci et al. 2020: binding to active RAC3; Erdmann et al. 2007; Lichter Konecki et al. 2006: no RAC3 directed GAP activity:)
OPHN1 (Amin et al. 2016: RAC3 directed GAP activity; Bagci et al. 2020: no binding to active RAC3)
PIK3R1 (Bagci et al. 2020: binding to active RAC3)
PIK3R2 (Bagci et al. 2020: binding to active RAC3)
SRGAP2 (Bagci et al. 2020: binding to active RAC3)
SYDE1 (Bagci et al. 2020: binding to active RAC3)

The following GAPs have been shown to not act on RAC3 or were shown to not bind to the constitutively active RAC3 mutant by Bagci et al. 2020:
ARHGAP12 (Bagci et al. 2020)
ARHGAP29 (Bagci et al. 2020)
ARHGAP31 (Bagci et al. 2020)
DLC1 (Amin et al. 2016)
MYO9A (Bagci et al. 2020)
MYO9B (Bagci et al. 2020)
STARD13 (Amin et al. 2016)
STARD8 (Amin et al. 2016)

Literature References
PubMed ID Title Journal Year
27481945 Deciphering the Molecular and Functional Basis of RHOGAP Family Proteins: A SYSTEMATIC APPROACH TOWARD SELECTIVE INACTIVATION OF RHO FAMILY PROTEINS

Amin, E, Jaiswal, M, Derewenda, U, Reis, K, Nouri, K, Koessmeier, KT, Aspenström, P, Somlyo, AV, Dvorsky, R, Ahmadian, MR

J. Biol. Chem. 2016
26628301 Inhibitory effects of Arhgap6 on cervical carcinoma cells

Li, J, Liu, Y, Yin, Y

Tumour Biol. 2016
17765681 A role of the Lowe syndrome protein OCRL in early steps of the endocytic pathway

Erdmann, KS, Mao, Y, McCrea, HJ, Zoncu, R, Lee, S, Paradise, S, Modregger, J, Biemesderfer, D, Toomre, D, De Camilli, P

Dev. Cell 2007
16777452 The effect of missense mutations in the RhoGAP-homology domain on ocrl1 function

Lichter-Konecki, U, Farber, LW, Cronin, JS, Suchy, SF, Nussbaum, RL

Mol. Genet. Metab. 2006
31871319 Mapping the proximity interaction network of the Rho-family GTPases reveals signalling pathways and regulatory mechanisms

Bagci, H, Sriskandarajah, N, Robert, A, Boulais, J, Elkholi, IE, Tran, V, Lin, ZY, Thibault, MP, Dubé, N, Faubert, D, Hipfner, DR, Gingras, AC, Cote, JF

Nat. Cell Biol. 2020
9252344 Characterization of RAC3, a novel member of the Rho family

Haataja, L, Groffen, J, Heisterkamp, N

J. Biol. Chem. 1997
27713499 Disruption of ArhGAP15 results in hyperactive Rac1, affects the architecture and function of hippocampal inhibitory neurons and causes cognitive deficits

Zamboni, V, Armentano, M, Sarò, G, Ciraolo, E, Ghigo, A, Germena, G, Umbach, A, Valnegri, P, Passafaro, M, Carabelli, V, Gavello, D, Bianchi, V, D'Adamo, P, de Curtis, I, El-Assawi, N, Mauro, A, Priano, L, Ferri, N, Hirsch, E, Merlo, GR

Sci Rep 2016
Participants
Participates
Catalyst Activity

GTPase activator activity of RAC3 GAPs [cytosol]

Orthologous Events
Authored
Reviewed
Created
Cite Us!