Reactome | RAC1 GEFs activate RAC1

RAC1 GEFs activate RAC1

Stable Identifier

R-HSA-9013143

Type

Reaction [transition]

Species

Homo sapiens

Compartment

plasma membrane, cytosol

ReviewStatus

5/5

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The following guanine nucleotide exchange factors (GEFs) were shown to bind RAC1 and catalyze GDP to GTP exchange on RAC1, resulting in formation of the active RAC1:GTP complex (the high throughput study by Bagci et al. examined binding of GEFs to inactive RAC1 mutant without testing for RAC1-directed GEF activity and is cited as supporting evidence):
DOCK3 (Namekata et al. 2004, Yang et al. 2012, Namekata et al. 2012; Müller et al. 2020)
FGD5 (Müller et al. 2020)
GNA13 (Radhika et al. 2004)
PLEKHG6 (Müller et al. 2020)
PREX1 (Yoshizawa et al. 2005; Dong et al. 2005; Marei et al. 2016; Jaiswal et al. 2013; Müller et al. 2020)
PREX2 (Joseph and Norris 2005; Müller et al. 2020)
RASGRF2 (Schwechter et al. 2013; Müller et al. 2020)
SWAP70 (Shinohara et al. 2002; Gupta et al. 2003; Baranov et al. 2016; Bagci et al. 2020: binding to inactive RAC1)
TIAM2 (Matsuo et al. 2002; Müller et al. 2020)
TRIO (Debant et al. 1996; Moshfegh et al. 2014; Peurois et al. 2017; Jaiswal et al. 2013: RAC1-directed GEF acivity of the N-terminal GEF1 domain of TRIO; Müller et al. 2020: RAC1-directed GEF activity of the full-length TRIO; Bagci et al. 2020: binding of full-length TRIO to inactive RAC1)
VAV1 (Teramoto et al. 1997; Heo et al. 2005; Aghazadeh et al. 2000; Crespo et al. 1997; Müller et al. 2020)
VAV2 (Itoh et al. 2008; Aoki et al. 2005; Heo et al. 2005; Jaiswal et al. 2013; Müller et al. 2020; Bagci et al. 2020: binding to inactive RAC1)

The following GEFs were shown to bind and activate RAC1 in some but not all studies and are annotated as candidate RAC1 GEFs (or were shown, in the high throughput screen by Bagci et al. 2020, to bind to nucleotide free RAC1 mutant, as indicated):
ABR (Chuang et al. 1995: RAC1-directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity)
ALS2 (Topp et al. 2004, Kanekura et al. 2005, Topp et al. 2005: RAC1 directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity)
ARHGEF4 (Itoh et al. 2008: RAC1 directed GEF activity; Anderson and Hamann 2012, Gotthardt and Ahmadian 2007, Jaiswal et al. 2013, Müller et al. 2020: no RAC1 directed GEF activity)
ARHGEF5 (Xie et al. 2005: RAC1 directed GEF activity; Wang et al. 2009, Müller et al. 2020: no RAC1 directed GEF activity; Bagci et al. 2020: no binding to inactive RAC1)
ARHGEF6 (Manser et al. 1998, Ramakers et al. 2012: RAC1 directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity)
ARHGEF7 (Ten Klooster et al. 2006, Manser et al. 1998: RAC1 directed GEF activity; Bagci et al. 2020: binding to inactive RAC1; Müller et al. 2020: no RAC1 directed GEF activity)
ARHGEF10 (Müller et al. 2020: RAC1 directed GEF activity; Mohl et al. 2006: no RAC1 directed GEF activity)
ARHGEF11 (Bagci et al. 2020: binding to inactive RAC1; Rümenapp et al. 1999; Jaiswal et al. 2011; Jaiswal et al. 2013; Müller et al. 2020: no RAC1 directed GEF activity:)
ARHGEF15 (Fukushima et al. 2016: RAC1 directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity)
ARHGEF18 (Niu et al. 2003: RAC1 directed GEF activity; Blomquist et al. 2000, Müller et al. 2020: no RAC1 directed GEF activity)
ARHGEF19 (Wang et al. 2004: RAC1 directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity)
ARHGEF25 (Guo et al. 2003: RAC1 directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity)
ARHGEF39 (Zhou et al. 2018: RAC1 directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity)
BCR (Chuang et al. 1995: RAC1 directed GEF activity; Bagci et al. 2020 binding to inactive RAC1; Korus et al. 2002, Müller et al. 2020: no RAC1 directed GEF activity)
DEF6 (Mavrakis et al. 2004: activation of RAC1, GEF activity of DEF6 has not been examined in vitro)
DOCK1 (Cote and Vuori 2002, Li et al. 2003: RAC1 directed GEF activity; Bagci et al. 2020: binding to inactive RAC1; Müller et al. 2020: no RAC1 directed GEF activity)
DOCK2 (Kulkarni et al. 2011: RAC1 directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity)
DOCK4 (Abraham et al. 2015: RAC1-directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity)
DOCK5 (Omi et al. 2008, Vives et al. 2011, Ferrandez et al. 2017: RAC1 directed GEF activity; Bagci et al. 2020: binding to inactive RAC1; Müller et al. 2020: no RAC1 directed GEF activity)
DOCK6 (Miyamoto et al. 2007: RAC1 directed GEF activity; Bagci et al. 2020: binding to inactive RAC1; Müller et al. 2020: no RAC1 directed GEF activity)
DOCK7 (Kukimoto Niino et al. 2019, Yamauchi et al. 2008, Majewski et al. 2012, Zhou et al. 2013: RAC1 directed GEF activity; Bagci et al. 2020: binding to inactive RAC1; Müller et al. 2020: no RAC1 directed GEF activity)
DOCK8 (Wang et al. 2015: RAC1 directed GEF activity; Bagci et al. 2020: no binding to inactive RAC1; Müller et al. 2020: no RAC1 directed GEF activity)
DOCK9 (Bagci et al. 2020: binding to inactive RAC1; Kulkarni et al. 2011, Müller et al. 2020: no RAC1 directed GEF activity)
DOCK10 (Ruiz Lafuente et al. 2015, Müller et al. 2020: RAC1 directed GEF activity; Bagci et al. 2020: no binding to inactive RAC1)
DOCK11 (Bagci et al. 2020: binding to inactive RAC1; Lin et al. 2006, Müller et al. 2020: no RAC1 directed GEF activity)
ECT2 (Tatsumoto et al. 1999: RAC1 directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity; Bagci et al. 2020: no binding to inactive RAC1)
FARP1 (Cheadle and Biederer 2012: RAC1-directed GEF activity; Bagci et al. 2020: binding to inactive RAC1; Müller et al. 2020: no RAC1 directed GEF activity)
FARP2 (Kubo et al. 2002: RAC1-directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity)
KALRN (Penzes et al. 2003, Wu et al. 2013: RAC1-directed GEF activity when using the N-terminal GEF1 domain of KALRN; Müller et al. 2020: no RAC1 directed GEF activity when using full-length KALRN)
MCF2 (Jaiswal et al. 2013, Müller et al. 2020: RAC1 directed GEF activity; Reuther et al. 2001: no RAC1 directed GEF activity);
MCF2L (Bagci et al. 2020: binding to inactive RAC1; Whitehead et al. 1999; Jaiswal et al. 2013; Müller et al. 2020: no RAC1 directed GEF activity)
NGEF (Zhang et al. 2007: RAC1 directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity; Bagci et al. 2020: no binding to inactive RAC1)
PLEKHG1 (Abiko et al. 2015: RAC1 directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity; Bagci et al. 2020: no binding to inactive RAC1)
PLEKHG2 (Ueda et al. 2008: RAC1 directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity; Bagci et al. 2020: no binding to inactive RAC1)
PLEKHG3 (Nguyen et al. 2016: RAC1-directed GEF activity; Bagci et al. 2020: binding to inactive RAC1; Müller et al. 2020: no RAC1 directed GEF activity)
PLEKHG4 (Gupta et al. 2013: RAC1 directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity; Bagci et al. 2020: no binding to inactive RAC1)
SOS1 (Nimnual et al. 1998: RAC1 directed GEF activity; Itoh et al. 2008, Müller et al. 2020: no RAC1 directed GEF activity)
SOS2 (Nimnual et al. 1998: RAC1 directed GEF activity; Itoh et al. 2008, Müller et al. 2020: no RAC1 directed GEF activity)
SPATA13 (Kawasaki et al. 2007, Bristow et al. 2009: RAC1 directed GEF activity; Hamann et al. 2007, Müller et al. 2020: no RAC1 directed GEF activity)
TIAM1 (Itoh et al. 2008, Michiels et al. 1995, Haeusler et al. 2003, Jaiswal et al. 2013, Müller et al. 2020: RAC1 directed GEF activity; Bagci et al. 2020: no binding to inactive RAC1)
VAV3 (Movilla and Bustello 1999, Sachdev et al. 2002, Aoki et al. 2005: RAC1-directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity)

The following GEFs do not act on RAC1 or were shown to not bind to nucleotide-free RAC1 in the study by Bagci et al. 2020, as indicated:
AKAP13 (Zheng et al. 1995; Müller et al. 2020; Bagci et al. 2020: no binding to inactive RAC1)
ARHGEF1 (Hart et al. 1996; Jaiswal et al. 2013; Jaiswal et al. 2011; Müller et al. 2020; Bagci et al. 2020: no binding to inactive RAC1)
ARHGEF2 (Krendel et al. 2002; Müller et al. 2020; Bagci et al. 2020: no binding to inactive RAC1)
ARHGEF3 (Arthur et al. 2002; Müller et al. 2020)
ARHGEF9 (Reid et al. 1999; Jaiswal et al. 2013; Müller et al. 2020)
ARHGEF10L (Winkler et al. 2005; Müller et al. 2020)
ARHGEF12 (Reuther et al. 2001; Jaiswal et al. 2013; Jaiswal et al. 2011; Müller et al. 2020; Bagci et al. 2020: no binding to inactive RAC1)
ARHGEF16 (Hiramoto Yamaki et al. 2010; Müller et al. 2020; Bagci et al. 2020: no binding to inactive RAC1)
ARHGEF17 (Rümenapp et al. 2002; Müller et al. 2020; Bagci et al. 2020: no binding to inactive RAC1)
ARHGEF26 (Ellerbroek et al. 2004; Müller et al. 2020; Bagci et al. 2020: no binding to inactive RAC1)
ARHGEF28 (van Horck et al. 2001; Jaiswal et al. 2013; Jaiswal et al. 2011; Müller et al. 2020)
ARHGEF40 (Curtis et al. 2004; Müller et al. 2020; Bagci et al. 2020: no binding to inactive RAC1)
DNMBP (Jaiswal et al. 2013; Müller et al. 2020; Bagci et al. 2020: no binding to inactive RAC1)
ECT2L (Müller et al. 2020)
FGD1 (Olson et al. 1996; Müller et al. 2020)
FGD2 (Huber et al. 2008; Müller et al. 2020)
FGD3 (Müller et al. 2020)
FGD4 (Umikawa et al. 1999; Müller et al. 2020)
FGD6 (Müller et al. 2020)
ITSN1 (Hussain et al. 2001; Jaiswal et al. 2013; Müller et al. 2020)
ITSN2 (Müller et al. 2020)
MCF2L2 (Müller et al. 2020)
NET1 (Alberts and Treisman 1998; Müller et al. 2020)
OBSCN (Ford Speelman et al. 2009)
PLEKHG4B (Müller et al. 2020)
PLEKHG5 (De Toledo et al. 2004; Müller et al. 2020)
PLEKHG7 (Müller et al. 2020)
RASGRF1 (Müller et al. 2020)

Literature References

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