TRAPPC complexes exchange GTP for GDP on RAB1

Stable Identifier
Reaction [transition]
Homo sapiens
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RAB1 is involved in COPII-mediated anterograde traffic from the endoplasmic reticulum to the ERGIC (ER-Golgi intermediate compartment) and in early steps of the macroautophagy pathway (reviewed in Szul and Sztul, 2011; Sandoval and Simmen, 2012; Lord et al, 2013; Yang et al, 2016; Lamb et al, 2016; Kim et al, 2016; Ao et al, 2014). RAB1 nucleotide exchange is stimulated in these pathways by the GEF activity of the multisubunit TRAPPC complexes II and III, respectively (reviewed in Brunet and Sacher, 2014; Kim et al, 2016). Note that the separate existence of a TRAPPCI complex is not clearly established in human cells (Barrowman et al, 2010; Bassik et al, 2013; reviewed in Brunet and Sacher, 2014; Kim et al, 2016). TRAPPCII is recruited to ER-derived vesicles by virtue of an interaction between the TRAPPCII component TRAPPC3 and the COPII coat protein SEC23 (Wang et al, 2000; Cai et al, 2007; Cai et al, 2008; Yamasaki et al, 2009; Lord et al, 2011; reviewed in Brunet and Sacher, 2014; Ishida et al, 2016). Interaction of TRAAPPCII with RAB1:GDP promotes release of GDP, allowing GTP to bind, and precludes the interaction of RAB1 with GDI and CHM proteins. Protein protein interactions involving activated RAB1:GTP help dock the ER-derived vesicle on the cis-Golgi membrane (reviewed in Lord et al, 2013). In the macroautophagy pathway, RAB1 and the TRAPPCIII complex play a role in the formation of the pre-autophagosomal structure (PAS) and contribute to the localization of ATG9, a key nucleator of autophagosome formation (Lynch-Day et al, 2010; Winslow et al, 2010; Zoppino et al, 2010; Mochizuki et al, 2013; Lamb et al, 2016; reviewed in Kim et al, 2016).
Literature References
PubMed ID Title Journal Year
19656848 mTrs130 is a component of a mammalian TRAPPII complex, a Rab1 GEF that binds to COPI-coated vesicles

Klumperman, J, Oorschot, V, Ferro-Novick, S, Yu, S, Yamasaki, A, Meerloo, T, Menon, S, Satoh, A, Barrowman, J

Mol. Biol. Cell 2009
17287728 TRAPPI tethers COPII vesicles by binding the coat subunit Sec23

Fu, C, Ferro-Novick, S, Yu, S, Cai, H, Menon, S, Lazarova, D, Cai, Y, Hay, JC, Reinisch, K

Nature 2007
22013193 COPII and COPI traffic at the ER-Golgi interface

Sztul, E, Szul, T

Physiology (Bethesda) 2011
23176493 Rab proteins of the endoplasmic reticulum: functions and interactors

Simmen, T, Ortiz Sandoval, C

Biochem. Soc. Trans. 2012
23378591 The highly conserved COPII coat complex sorts cargo from the endoplasmic reticulum and targets it to the golgi

Ferro-Novick, S, Miller, EA, Lord, C

Cold Spring Harb Perspect Biol 2013
25341920 Rab proteins and the compartmentalization of the endosomal system

Wandinger-Ness, A, Zerial, M

Cold Spring Harb Perspect Biol 2014
11038176 TRAPP stimulates guanine nucleotide exchange on Ypt1p

Wang, W, Ferro-Novick, S, Sacher, M

J. Cell Biol. 2000
24917561 In sickness and in health: the role of TRAPP and associated proteins in disease

Sacher, M, Brunet, S

Traffic 2014
18585354 The structural basis for activation of the Rab Ypt1p by the TRAPP membrane-tethering complexes

Sclafani, A, Fu, C, Ferro-Novick, S, Reinisch, KM, Cai, H, Menon, S, Lazarova, D, De La Cruz, EM, Cai, Y, Chin, HF, Rodgers, DW

Cell 2008
21532587 Sequential interactions with Sec23 control the direction of vesicle traffic

Ghassemian, M, Ghosh, P, Bhandari, D, Ferro-Novick, S, Menon, S, Nycz, D, Lord, C, Hay, J

Nature 2011
27246931 Multiple Types of Guanine Nucleotide Exchange Factors (GEFs) for Rab Small GTPases

Oguchi, ME, Fukuda, M, Ishida, M

Cell Struct. Funct. 2016
27041585 Rab1 in cell signaling, cancer and other diseases

Li, XX, Zhang, YJ, Zheng, XF, Rodriguez-Rodriguez, L, Xiang, MQ, Wang, HY, Yang, XZ

Oncogene 2016
Catalyst Activity

guanyl-nucleotide exchange factor activity of TRAPPCs [cytosol]

Orthologous Events
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