NMRK1 phosphorylates NR to yield NMN

Stable Identifier
Homo sapiens
NR + ATP(4-) => NMN + ADP(3-) + H+
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NMRK1 (nicotinamide riboside kinase 1) catalyzes the reaction of NR (N-ribosylnicotinamide) and ATP to yield NMN (beta-nicotinamide D-ribonucleotide), ADP, and H+. The enzyme is also active with GTP as a phosphate donor (not annotated here) (Bieganowsky & Brenner 2004; Sasiak & Saunders 1996; Tempel et al. 2007). The reaction is annotated with ATP(4-), the major ionized form of ATP at pH 7.2 (Stockbridge & Wolfenden 2009), as the phosphate donor. NMRK1 is a cytosolic enzyme (Nikiforov et al. 2011).

Literature References
PubMed ID Title Journal Year
15137942 Discoveries of nicotinamide riboside as a nutrient and conserved NRK genes establish a Preiss-Handler independent route to NAD+ in fungi and humans

Bieganowski, P, Brenner, C

Cell 2004
8809081 Purification and properties of a human nicotinamide ribonucleoside kinase

Saunders, PP, Sasiak, K

Arch. Biochem. Biophys. 1996
21504897 Pathways and subcellular compartmentation of NAD biosynthesis in human cells: from entry of extracellular precursors to mitochondrial NAD generation

Ziegler, M, Nikiforov, A, Dölle, C, Niere, M

J. Biol. Chem. 2011
17914902 Nicotinamide riboside kinase structures reveal new pathways to NAD+

Sauve, AA, Park, HW, Yang, T, Belenky, P, Seidle, HF, Tempel, W, Nedyalkova, L, Wojcik, M, Rabeh, WM, Bogan, KL, Brenner, C

PLoS Biol. 2007
19531469 The intrinsic reactivity of ATP and the catalytic proficiencies of kinases acting on glucose, N-acetylgalactosamine, and homoserine: a thermodynamic analysis

Wolfenden, R, Stockbridge, RB

J. Biol. Chem. 2009
Catalyst Activity

ribosylnicotinamide kinase activity of NMRK1 [cytosol]

Orthologous Events
Cross References
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