Activation of ubiquitin by UBA1 proceeds through 3 steps: adenylation of ubiquitin, conjugation of ubiquitin from adenyl-ubiquitin to an internal cysteine residue of UBA1, and adenylation of a second molecule of ubiquitin. In the first step the adenylation module of UBA1 catalyzes the acyl-adenylation of the C-terminal glycine of ubiquitin (Jin et al. 2007, inferred from the rabbit homologue in Haas et al. 1982, Hershko et al. 1983, reviewed in Groettrup et al. 2008).
Li, X, Gygi, SP, Jin, J, Harper, JW
Hofmann, K, Pelzer, C, Groettrup, M, Schmidtke, G
Rose, IA, Hershko, A, Haas, AL, Warms, JV
Ciechanover, A, Elias, S, Heller, H, Hershko, A
ubiquitin activating enzyme activity of UBA1 [cytosol]
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