Reactome | UBA1 adenylates ubiquitin in the cytosol

UBA1 adenylates ubiquitin in the cytosol

Stable Identifier

R-HSA-8852134

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol

ReviewStatus

5/5

Locations in the PathwayBrowser

Expand all

General

SBML | | PDF

SVG | | PPTX | SBGN

UBA1 adenylates ubiquitin in the cytosol

Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

Activation of ubiquitin by UBA1 proceeds through 3 steps: adenylation of ubiquitin, conjugation of ubiquitin from adenyl-ubiquitin to an internal cysteine residue of UBA1, and adenylation of a second molecule of ubiquitin. In the first step the adenylation module of UBA1 catalyzes the acyl-adenylation of the C-terminal glycine of ubiquitin (Jin et al. 2007, inferred from the rabbit homologue in Haas et al. 1982, Hershko et al. 1983, reviewed in Groettrup et al. 2008).

Literature References

PubMed ID	Title	Journal	Year
17597759	Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging Li, X, Gygi, SP, Jin, J, Harper, JW	Nature	2007
18353650	Activating the ubiquitin family: UBA6 challenges the field Hofmann, K, Pelzer, C, Groettrup, M, Schmidtke, G	Trends Biochem. Sci.	2008
6277905	Ubiquitin-activating enzyme. Mechanism and role in protein-ubiquitin conjugation Rose, IA, Hershko, A, Haas, AL, Warms, JV	J. Biol. Chem.	1982
6305978	Components of ubiquitin-protein ligase system. Resolution, affinity purification, and role in protein breakdown Ciechanover, A, Elias, S, Heller, H, Hershko, A	J. Biol. Chem.	1983