Reactome | (2'-deoxy)cytidine + ATP => (d)CMP + ADP (DCK)

(2'-deoxy)cytidine + ATP => (d)CMP + ADP (DCK)

Stable Identifier

R-HSA-73598

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol

Synonyms

phosphorylation of 2'-Deoxycytidine to 2'-Deoxycytidine 5'-phosphate

ReviewStatus

5/5

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(2'-deoxy)cytidine + ATP => (d)CMP + ADP (DCK)

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Cytosolic deoxycytidine kinase (DCK) catalyzes the reactions of cytidine or deoxycytidine with ATP to form CMP or dCMP and ADP. The enzyme is a homodimer (Sabini et al. 2003). Although a chimeric deoxycytidine kinase - green fluorescent protein expressed at high levels in cultured cells localized to nuclei, endogenous protein is primarily cytosolic (Hatzis et al. 1998). Despite its name, the enzyme has a broad substrate specificity, acting on cytidine, deoxycytidine, deoxyguanosine, and deoxyadenosine (Bohman and Eriksson 1988; Datta et al. 1989a, b; Sarup et al. 1989; Usova and Eriksson 2002). While ATP functions efficiently as a phosphate donor, other nucleoside triphosphates, notably UTP, function efficiently as phosphate donors in vitro and may function in this way in vivo as well (Shewach et al. 1992).

Literature References

PubMed ID	Title	Journal	Year
2844225	Deoxycytidine kinase from human leukemic spleen: preparation and characterization of the homogeneous enzyme Bohman, C, Eriksson, S	Biochemistry	1988
12808445	Structure of human dCK suggests strategies to improve anticancer and antiviral therapy Monnerjahn, C, Konrad, M, Sabini, E, Ort, S, Lavie, A	Nat Struct Biol	2003
2542307	Kinetic properties and inhibition of human T lymphoblast deoxycytidine kinase Datta, NS, Fox, IH, Shewach, DS, Mitchell, BS	J Biol Chem	1989
9804782	The intracellular localization of deoxycytidine kinase Talianidis, I, Jullig, M, Petrakis, TG, Hatzis, P, Al-Madhoon, AS, Eriksson, S	J Biol Chem	1998
2539852	Human T-lymphoblast deoxycytidine kinase: purification and properties Datta, NS, Fox, IH, Hurley, MC, Shewach, DS, Mitchell, BS	Biochemistry	1989
2548513	Regulation of purine deoxynucleoside phosphorylation by deoxycytidine kinase from human leukemic blast cells Fridland, A, Johnson, MA, Verhoef, V, Sarup, JC	Biochem Pharmacol	1989
12429345	Identification of residues involved in the substrate specificity of human and murine dCK Usova, EV, Eriksson, S	Biochem Pharmacol	2002
1406603	Nucleotide specificity of human deoxycytidine kinase Hertel, L, Reynolds, KK, Shewach, DS	Mol Pharmacol	1992
1996353	Cloning and expression of human deoxycytidine kinase cDNA Ginsberg, D, Datta, NS, Fox, IH, Ashcraft, E, Gribbin, D, Chottiner, EG, Shewach, DS, Mitchell, BS	Proc Natl Acad Sci USA	1991