(2'-deoxy)cytidine + ATP => (d)CMP + ADP (DCK)

Stable Identifier
Reaction [transition]
Homo sapiens
phosphorylation of 2'-Deoxycytidine to 2'-Deoxycytidine 5'-phosphate
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Cytosolic deoxycytidine kinase (DCK) catalyzes the reactions of cytidine or deoxycytidine with ATP to form CMP or dCMP and ADP. The enzyme is a homodimer (Sabini et al. 2003). Although a chimeric deoxycytidine kinase - green fluorescent protein expressed at high levels in cultured cells localized to nuclei, endogenous protein is primarily cytosolic (Hatzis et al. 1998). Despite its name, the enzyme has a broad substrate specificity, acting on cytidine, deoxycytidine, deoxyguanosine, and deoxyadenosine (Bohman and Eriksson 1988; Datta et al. 1989a, b; Sarup et al. 1989; Usova and Eriksson 2002). While ATP functions efficiently as a phosphate donor, other nucleoside triphosphates, notably UTP, function efficiently as phosphate donors in vitro and may function in this way in vivo as well (Shewach et al. 1992).

Literature References
PubMed ID Title Journal Year
2844225 Deoxycytidine kinase from human leukemic spleen: preparation and characterization of the homogeneous enzyme

Bohman, C, Eriksson, S

Biochemistry 1988
12808445 Structure of human dCK suggests strategies to improve anticancer and antiviral therapy

Monnerjahn, C, Konrad, M, Sabini, E, Ort, S, Lavie, A

Nat Struct Biol 2003
2542307 Kinetic properties and inhibition of human T lymphoblast deoxycytidine kinase

Datta, NS, Fox, IH, Shewach, DS, Mitchell, BS

J Biol Chem 1989
9804782 The intracellular localization of deoxycytidine kinase

Talianidis, I, Jullig, M, Petrakis, TG, Hatzis, P, Al-Madhoon, AS, Eriksson, S

J Biol Chem 1998
2539852 Human T-lymphoblast deoxycytidine kinase: purification and properties

Datta, NS, Fox, IH, Hurley, MC, Shewach, DS, Mitchell, BS

Biochemistry 1989
2548513 Regulation of purine deoxynucleoside phosphorylation by deoxycytidine kinase from human leukemic blast cells

Fridland, A, Johnson, MA, Verhoef, V, Sarup, JC

Biochem Pharmacol 1989
12429345 Identification of residues involved in the substrate specificity of human and murine dCK

Usova, EV, Eriksson, S

Biochem Pharmacol 2002
1406603 Nucleotide specificity of human deoxycytidine kinase

Hertel, L, Reynolds, KK, Shewach, DS

Mol Pharmacol 1992
1996353 Cloning and expression of human deoxycytidine kinase cDNA

Ginsberg, D, Datta, NS, Fox, IH, Ashcraft, E, Gribbin, D, Chottiner, EG, Shewach, DS, Mitchell, BS

Proc Natl Acad Sci USA 1991
Catalyst Activity

nucleoside kinase activity of DCK dimer [cytosol]

Orthologous Events
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