Phosphorylation and activation of CHEK2 by ATM

Stable Identifier
Reaction [transition]
Homo sapiens
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Activated ATM phosphorylates CHEK2 (CHK2, Cds1) on threonine residue T68 (Matsuoka et al. 2000, Melchionna et al. 2000). The presence of BRCA1 and TP53BP1 positively regulates ATM-mediated phosphorylation of CHEK2 (Wang et al. 2002, Foray et al. 2003). ATM-mediated phosphorylation causes formation of CHEK2 dimers and dissociation of CHEK2 from chromatin (Li and Stern 2005).
Literature References
PubMed ID Title Journal Year
11025670 Threonine 68 is required for radiation-induced phosphorylation and activation of Cds1

Melchionna, R, Chen, XB, McGowan, CH, Blasina, A

Nat. Cell Biol. 2000
16150728 DNA damage regulates Chk2 association with chromatin

Stern, DF, Li, J

J. Biol. Chem. 2005
12364621 53BP1, a mediator of the DNA damage checkpoint.

Elledge, SJ, Carpenter, PB, Wang, B, Matsuoka, S

Science 2002
10973490 Ataxia telangiectasia-mutated phosphorylates Chk2 in vivo and in vitro

Shiloh, Y, Tamai, K, Ogawa, A, Elledge, SJ, Rotman, G, Matsuoka, S

Proc Natl Acad Sci U S A 2000
12773400 A subset of ATM- and ATR-dependent phosphorylation events requires the BRCA1 protein.

Ashworth, A, Jeggo, P, Perricaudet, M, Gabriel, A, Randrianarison, V, Foray, N, Marot, D

EMBO J 2003
Catalyst Activity

protein serine/threonine kinase activity of DNA DNA DSBs:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:K63PolyUb-K14,K16,p-S139-H2AFX,Me2K21-HIST1H4A-Nucleosome:p-5T-MDC1:p-S102-WHSC1:RNF8:Zn2+:SUMO1:p-T4827-HERC2:UBE2N:UBE2V2:RNF168:PIAS4:p-S25,S1778-TP53BP1:p-4S,2T-BRCA1-A complex:CHEK2 [nucleoplasm]

Orthologous Events
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