Reactome | DNA strand elongation

DNA strand elongation

Stable Identifier

R-HSA-69190

DOI

10.3180/REACT_932.2

Type

Pathway

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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Accurate and efficient genome duplication requires coordinated processes to replicate two template strands at eucaryotic replication forks. Knowledge of the fundamental reactions involved in replication fork progression is derived largely from biochemical studies of the replication of simian virus and from yeast genetic studies. Since duplex DNA forms an anti-parallel structure, and DNA polymerases are unidirectional, one of the new strands is synthesized continuously in the direction of fork movement. This strand is designated as the leading strand. The other strand grows in the direction away from fork movement, and is called the lagging strand. Several specific interactions among the various proteins involved in DNA replication underlie the mechanism of DNA synthesis, on both the leading and lagging strands, at a DNA replication fork. These interactions allow the replication enzymes to cooperate in the replication process (Hurwitz et al 1990; Brush et al 1996; Ayyagari et al 1995; Budd & Campbell 1997; Bambara et al 1997).

Literature References

PubMed ID	Title	Journal	Year
1536007	A yeast chromosomal origin of DNA replication defined by multiple functional elements. Marahrens, Y, Stillman, B	Science	1992
7623835	A mutational analysis of the yeast proliferating cell nuclear antigen indicates distinct roles in DNA replication and DNA repair. Ayyagari, R, Impellizzeri, KJ, Yoder, BL, Gary, SL, Burgers, PM	Mol Cell Biol	1995
8594377	Identification of eukaryotic DNA replication proteins using simian virus 40 in vitro replication system. Brush, GS, Kelly, TJ, Stillman, B	Methods Enzymol	1996
1976634	The in vitro replication of DNA containing the SV40 origin. Hurwitz, J, Kwong, AD, Lee, SH	J Biol Chem	1990
9081985	Enzymes and reactions at the eukaryotic DNA replication fork. Bambara, RA, Murante, RS, Henricksen, LA	J Biol Chem	1997