Active ARF recruits coatomer to the Golgi

Stable Identifier
Reaction [binding]
Homo sapiens
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Activation of ARF is tightly correlated to recruitment of the COPI coat (Donaldson et al, 1991; Serafini et al, 1991; Donaldson et el, 1992; Palmer et al, 1993; reveiwed in Szul and Sztul, 2011). Studies in yeast and in mammalian cells support a direct interaction between the GTPase and components of the COPI coat (Zhao et al, 1997; Zhao et al, 1999; Zhao et al, 2006; Eugster et al, 2000; Sun et al, 2007; Yu et al, 2012; Harter and Wieland, 1998; Bethune et al, 2006; reviewed in Popoff et al, 2011). The COPI coat consists of 7 subunits arranged in 2 subcomplexes. The inner coat is made up of a tetrameric complex consisting of the beta, gamma, zeta and delta COPI subunits, while the outer coat is a trimer consisting of the alpha, beta prime and epsilon subunits (Eugster et al, 2000; Waters et al, 1991). Both of the zeta and gamma subunits have 2 isoforms with different subcellular locations, suggesting that different COPI coats may mediate different steps of the secretory pathway (Moelleken et al, 2007). Unlike the case for COPII or clathrin coats, all components of the COPI coat are recruited simultaneously as a preformed heptameric complex (Hara-Kuge et al, 1994).

Literature References
PubMed ID Title Journal Year
10318838 GTP-dependent binding of ADP-ribosylation factor to coatomer in close proximity to the binding site for dilysine retrieval motifs and p23

Zhao, L, Helms, JB, Brunner, J, Wieland, FT

J. Biol. Chem. 1999
17360540 Differential localization of coatomer complex isoforms within the Golgi apparatus

Moelleken, J, Malsam, J, Betts, MJ, Movafeghi, A, Reckmann, I, Meissner, I, Hellwig, A, Russell, RB, Söllner, T, Brugger, B, Wieland, FT

Proc. Natl. Acad. Sci. U.S.A. 2007
1680566 ADP-ribosylation factor is a subunit of the coat of Golgi-derived COP-coated vesicles: a novel role for a GTP-binding protein

Serafini, T, Orci, L, Amherdt, M, Brunner, M, Kahn, RA, Rothman, JE

Cell 1991
22304919 A structure-based mechanism for Arf1-dependent recruitment of coatomer to membranes

Yu, X, Breitman, M, Goldberg, J

Cell 2012
16926190 GBF1, a cis-Golgi and VTCs-localized ARF-GEF, is implicated in ER-to-Golgi protein traffic

Zhao, X, Claude, A, Chun, J, Shields, DJ, Presley, JF, Melançon, P

J. Cell. Sci. 2006
1631136 ADP-ribosylation factor, a small GTP-binding protein, is required for binding of the coatomer protein beta-COP to Golgi membranes

Donaldson, JG, Cassel, D, Kahn, RA, Klausner, RD

Proc. Natl. Acad. Sci. U.S.A. 1992
22013193 COPII and COPI traffic at the ER-Golgi interface

Szul, T, Sztul, E

Physiology (Bethesda) 2011
21844168 COPI budding within the Golgi stack

Popoff, V, Adolf, F, Brugger, B, Wieland, F

Cold Spring Harb Perspect Biol 2011
1898986 'Coatomer': a cytosolic protein complex containing subunits of non-clathrin-coated Golgi transport vesicles

Waters, MG, Serafini, T, Rothman, JE

Nature 1991
1957170 Binding of ARF and beta-COP to Golgi membranes: possible regulation by a trimeric G protein

Donaldson, JG, Kahn, RA, Lippincott-Schwartz, J, Klausner, RD

Science 1991
9751720 A single binding site for dilysine retrieval motifs and p23 within the gamma subunit of coatomer

Harter, C, Wieland, FT

Proc. Natl. Acad. Sci. U.S.A. 1998
8132710 En bloc incorporation of coatomer subunits during the assembly of COP-coated vesicles

Hara-Kuge, S, Kuge, O, Orci, L, Amherdt, M, Ravazzola, M, Wieland, FT, Rothman, JE

J. Cell Biol. 1994
16940185 Coatomer, the coat protein of COPI transport vesicles, discriminates endoplasmic reticulum residents from p24 proteins

Béthune, J, Kol, M, Hoffmann, J, Reckmann, I, Brugger, B, Wieland, F

Mol. Cell. Biol. 2006
9114004 Direct and GTP-dependent interaction of ADP ribosylation factor 1 with coatomer subunit beta

Zhao, L, Helms, JB, Brugger, B, Harter, C, Martoglio, B, Graf, R, Brunner, J, Wieland, FT

Proc. Natl. Acad. Sci. U.S.A. 1997
8505331 Binding of coatomer to Golgi membranes requires ADP-ribosylation factor

Palmer, DJ, Helms, JB, Beckers, CJ, Orci, L, Rothman, JE

J. Biol. Chem. 1993
17451557 Multiple and stepwise interactions between coatomer and ADP-ribosylation factor-1 (Arf1)-GTP

Sun, Z, Anderl, F, Fröhlich, K, Zhao, L, Hanke, S, Brugger, B, Wieland, F, Béthune, J

Traffic 2007
10921873 COP I domains required for coatomer integrity, and novel interactions with ARF and ARF-GAP

Eugster, A, Frigerio, G, Dale, M, Duden, R

EMBO J. 2000
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