GBF1 recruits ARF:GDP to the Golgi

Stable Identifier
Reaction [binding]
Homo sapiens
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GBF1 recruits inactive ARF:GDP complexes to the Golgi (Monetta et al, 2007). There are 5 known ARFs in the human cell. Class I members ARF1 and ARF 3 are expressed at high levels and broadly distributed through the secretory system, while Class II members ARF4 and 5 are expressed at lower levels. ARF6, the single Class III ARF, appears to function more specifically in endocytosis and actin dynamics (Chun et al, 2008; reviewed in D'Souza-Schorey and Chavrier, 2006; Szul and Sztul, 2011). GBF1 has been shown to activate ARF1, 4, and 5, but not ARF3, while single and pairwise knockdown of ARF1, 3, 4 and 5 suggests that no single ARF is responsible for any given step in the secretory pathway (Manolea et al, 2010; Volpicelli-Daley et al, 2005).

Literature References
PubMed ID Title Journal Year
22013193 COPII and COPI traffic at the ER-Golgi interface

Sztul, E, Szul, T

Physiology (Bethesda) 2011
18524849 Characterization of class I and II ADP-ribosylation factors (Arfs) in live cells: GDP-bound class II Arfs associate with the ER-Golgi intermediate compartment independently of GBF1

Presley, JF, Melançon, P, Chun, J, Dejgaard, SY, Shapovalova, Z

Mol. Biol. Cell 2008
20357002 Arf3 is activated uniquely at the trans-Golgi network by brefeldin A-inhibited guanine nucleotide exchange factors

Manolea, F, Chen, DW, Melançon, P, Summerfeldt, N, Clarke, I, Dacks, JB, Chun, J

Mol. Biol. Cell 2010
17429068 Rab1b interacts with GBF1 and modulates both ARF1 dynamics and COPI association

Slavin, I, Monetta, P, Romero, N, Alvarez, C

Mol. Biol. Cell 2007
16030262 Isoform-selective effects of the depletion of ADP-ribosylation factors 1-5 on membrane traffic

Volpicelli-Daley, LA, Zhang, CJ, Li, Y, Kahn, RA

Mol. Biol. Cell 2005
16633337 ARF proteins: roles in membrane traffic and beyond

Chavrier, P, D'Souza-Schorey, C

Nat. Rev. Mol. Cell Biol. 2006
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