Calmodulin (CALM1) is a ubiquitous key mediator of Ca2+-dependent signalling and is subject to regulatory post-translational modifications which can affect protein-protein interactions. CALM1 is frequently trimethylated at Lys-116 which can influence changes in growth and development and its activator properties with target enzymes. The cytosolic enzyme calmodulin-lysine N-methyltransferase (CAMKMT) catalyses the transfer of 3 methyl groups from high energy donor S-adenosyl-L-methionine (AdoMet) to lysine residue 116 in CALM1, forming a trimethylated protein (triMe-K116-CALM1) (Magnani et al. 2010).