Reactome | HAGH hydrolyses (R)-S-LGSH to GSH and LACT

HAGH hydrolyses (R)-S-LGSH to GSH and LACT

Stable Identifier

R-HSA-6783221

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol

ReviewStatus

5/5

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HAGH hydrolyses (R)-S-LGSH to GSH and LACT

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In the second step of the glyoxalase system, hydroxyacylglutathione hydrolase (HAGH) catalyses the hydrolysis of (R)-S-lactoylglutathione ((R)-S-LGSH) to glutathione (GSH) and D-lactate (D-LACT) (Ridderstrom et al. 1996). The HAGH gene can produce two forms of the protein, form 1 is mitochondrial whereas form 2 is cytosolic (Cordell et al. 2004). HAGH is monomeric but requires two Zn2+ ions for activity (Cameron et al. 1999). This reaction completes the detoxification of methylglyoxal, a reactive byproduct of pyruvate metabolism.

Literature References

PubMed ID	Title	Journal	Year
10508780	Crystal structure of human glyoxalase II and its complex with a glutathione thiolester substrate analogue Cameron, AD, Ridderström, M, Olin, B, Mannervik, B	Structure	1999
8550579	Molecular cloning, heterologous expression, and characterization of human glyoxalase II Ridderström, M, Saccucci, F, Hellman, U, Bergman, T, Principato, G, Mannervik, B	J. Biol. Chem.	1996
15117945	The Human hydroxyacylglutathione hydrolase (HAGH) gene encodes both cytosolic and mitochondrial forms of glyoxalase II Cordell, PA, Futers, TS, Grant, PJ, Pease, RJ	J. Biol. Chem.	2004