NSUN2 methylates cytidine-34, cytidine-48 of unspliced tRNA(Leu)(CAA)

Stable Identifier
Reaction [transition]
Homo sapiens
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NSUN2 transfers a methyl group from S-adenosylmethionine to the 5 positions of cytidine-34 and cytidine-48 in tRNA(Leu)(CAA) (Brzezicha et al. 2006, Auxilien et al. 2012, Squires et al. 2012, Khoddami and Cairns 2013). Methylation of cytidine-34 occurs on the uspliced precursor tRNA(Leu)(CAA) (Brzezicha et al. 2006, Auxilien et al. 2012); methylation of cytidine-48 occurs on either the spliced or unspliced tRNA(Leu)(CAA) (Auxilien et al. 2012).
Literature References
PubMed ID Title Journal Year
22995836 The human tRNA m (5) C methyltransferase Misu is multisite-specific

Auxilien, S, Golinelli-Pimpaneau, B, Guérineau, V, Szweykowska-Kulińska, Z

RNA Biol 2012
23604283 Identification of direct targets and modified bases of RNA cytosine methyltransferases

Cairns, BR, Khoddami, V

Nat. Biotechnol. 2013
22344696 Widespread occurrence of 5-methylcytosine in human coding and non-coding RNA

Humphreys, DT, Patel, HR, Squires, JE, Preiss, T, Sibbritt, T, Nousch, M, Suter, CM, Parker, BJ

Nucleic Acids Res. 2012
17071714 Identification of human tRNA:m5C methyltransferase catalysing intron-dependent m5C formation in the first position of the anticodon of the pre-tRNA Leu (CAA)

Brzezicha, B, Schmidt, M, Jarmolowski, A, Szweykowska-Kulinska, Z, Makalowska, I, Pienkowska, J

Nucleic Acids Res. 2006
Event Information
Go Biological Process
Catalyst Activity

tRNA (cytosine-5-)-methyltransferase activity of NSUN2 [nucleoplasm]

Inferred From
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