NSUN2 methylates cytidine-34, cytidine-48 of unspliced tRNA(Leu)(CAA)

Stable Identifier
R-HSA-6782388
Type
Reaction [transition]
Species
Homo sapiens
Compartment
nucleoplasm
ReviewStatus
5/5
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NSUN2 methylates cytidine-34, cytidine-48 of unspliced tRNA(Leu)(CAA)
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NSUN2 transfers a methyl group from S-adenosylmethionine to the 5 positions of cytidine-34 and cytidine-48 in tRNA(Leu)(CAA) (Brzezicha et al. 2006, Auxilien et al. 2012, Squires et al. 2012, Khoddami and Cairns 2013). Methylation of cytidine-34 occurs on the uspliced precursor tRNA(Leu)(CAA) (Brzezicha et al. 2006, Auxilien et al. 2012); methylation of cytidine-48 occurs on either the spliced or unspliced tRNA(Leu)(CAA) (Auxilien et al. 2012).
Literature References
PubMed ID Title Journal Year
22995836 The human tRNA m (5) C methyltransferase Misu is multisite-specific

Auxilien, S, Guérineau, V, Szweykowska-Kulińska, Z, Golinelli-Pimpaneau, B

RNA Biol 2012
23604283 Identification of direct targets and modified bases of RNA cytosine methyltransferases

Khoddami, V, Cairns, BR

Nat. Biotechnol. 2013
22344696 Widespread occurrence of 5-methylcytosine in human coding and non-coding RNA

Squires, JE, Patel, HR, Nousch, M, Sibbritt, T, Humphreys, DT, Parker, BJ, Suter, CM, Preiss, T

Nucleic Acids Res. 2012
17071714 Identification of human tRNA:m5C methyltransferase catalysing intron-dependent m5C formation in the first position of the anticodon of the pre-tRNA Leu (CAA)

Brzezicha, B, Schmidt, M, Makalowska, I, Jarmolowski, A, Pienkowska, J, Szweykowska-Kulinska, Z

Nucleic Acids Res. 2006
Participants
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Output
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Event Information
Go Biological Process
tRNA modification (0006400)
Catalyst Activity

tRNA (cytidine-5-)-methyltransferase activity of NSUN2 [nucleoplasm]

Physical Entity
NSUN2 [nucleoplasm] (Homo sapiens)
Activity
tRNA (cytidine-5-)-methyltransferase activity (GO:0016428)
Inferred From
NCL1 (TRM4) methylates cytidine-34 of unspliced tRNA(Leu) yielding 5-methylcytidine-34 (Saccharomyces cerevisiae)
Authored
May, B  (2015-06-06)
Reviewed
Levinger, L  (2015-08-11)
Motorin, Y  (2015-08-25)
Jarrous, N  (2015-10-24)
Created
May, B  (2015-06-07)
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