Reactome | Deposition of new CENPA-containing nucleosomes at the centromere

Deposition of new CENPA-containing nucleosomes at the centromere

Stable Identifier

R-HSA-606279

Type

Pathway

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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Deposition of new CENPA-containing nucleosomes at the centromere

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Eukaryotic centromeres are marked by a unique form of histone H3, designated CENPA in humans. In human cells newly synthesized CENPA is deposited in nucleosomes at the centromere during late telophase/early G1 phase of the cell cycle. Once deposited, nucleosomes containing CENPA remain stably associated with the centromere and are partitioned equally to daughter centromeres during S phase. A current model proposes that pre-existing CENPA at the centromere drives recruitment of new CENPA, however this has not been proved.
The deposition process requires at least 3 complexes: the Mis18 complex, HJURP complex, and the RSF complex. HJURP binds newly synthesized CENPA-H4 tetramers before deposition and brings them to the centromere for deposition in new CENPA-containing nucleosomes. The exact mechanism of deposition remains unknown.

Literature References

PubMed ID	Title	Journal	Year
19410545	HJURP is a cell-cycle-dependent maintenance and deposition factor of CENP-A at centromeres Daigo, Y, Lacoste, N, Dunleavy, EM, Tagami, H, Almouzni-Pettinotti, G, Nakatani, Y, Roche, D, Nakamura, Y, Ray-Gallet, D	Cell	2009
19660450	Epigenetic specification of centromeres by CENP-A Losada, A, Bernad, R, Sánchez, P	Exp Cell Res	2009
20080577	HJURP binds CENP-A via a highly conserved N-terminal domain and mediates its deposition at centromeres Ouararhni, K, Hamiche, A, Dimitrov, S, Shuaib, M	Proc Natl Acad Sci U S A	2010
17339380	Propagation of centromeric chromatin requires exit from mitosis Jansen, LE, Foltz, DR, Black, BE, Cleveland, DW	J Cell Biol	2007
19410539	Frodos found: Behold the CENP-a "Ring" bearers Zhang, W, Mellone, BG, Karpen, GH	Cell	2009
19410544	Centromere-specific assembly of CENP-a nucleosomes is mediated by HJURP Wood, S, Jansen, LE, Yates, JR 3rd, Foltz, DR, Black, BE, Bailey, AO, Cleveland, DW, Bassett, EA	Cell	2009
19590885	At the right place at the right time: novel CENP-A binding proteins shed light on centromere assembly Jansen, LE, Silva, MC	Chromosoma	2009
19629040	Focus on the centre: the role of chromatin on the regulation of centromere identity and function Moreno-Moreno, O, Torras-Llort, M, Azorín, F	EMBO J	2009