AUF1(hnRNP D0) dimers bind AU-rich element in 3' UTR of mRNA

Stable Identifier
Reaction [binding]
Homo sapiens
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AUF1 monomers form dimers which bind the U-rich sequences in AU-rich elements of mRNAs. Binding of the mRNA causes the dimers of AUF1 to form tetramers. Nonphosphorylated AUF1 isoform p40 causes the mRNA to form a rigid structure whereas p40 that is phosphorylated at serines 83 and 87 does not. This difference may cause nonphosphorylated p40 to fail to destabilize the bound mRNA.
Literature References
PubMed ID Title Journal Year
19074427 Similar regulation of human inducible nitric-oxide synthase expression by different isoforms of the RNA-binding protein AUF1

Kleinert, H, Schneider, RJ, Art, J, Knauer, S, Pont, A, Stauber, R, Pautz, A, Sadri, N, Heil, S, Altenhöfer, S, Linker, K, Schmidt, N

J Biol Chem 2009
10559216 Assembly of AUF1 oligomers on U-rich RNA targets by sequential dimer association

Brewer, G, Wilson, GM, Lu, H, Sun, Y

J Biol Chem 1999
17486099 Competitive binding of AUF1 and TIAR to MYC mRNA controls its translation

Brewer, G, Hu, Y, Liao, B

Nat Struct Mol Biol 2007
14976220 Roles of AUF1 isoforms, HuR and BRF1 in ARE-dependent mRNA turnover studied by RNA interference

Certa, U, Gross, B, Wegmueller, D, Raineri, I, Moroni, C

Nucleic Acids Res 2004
12819194 Phosphorylation of p40AUF1 regulates binding to A + U-rich mRNA-destabilizing elements and protein-induced changes in ribonucleoprotein structure

Ysla, RM, Brewer, G, Villanueva-Feliciano, EC, Charles, S, Brewer, B, Suarez, Y, Wilson, GM, Sinha, S, Lu, J, Sutphen, K

J Biol Chem 2003
8246982 Purification, characterization, and cDNA cloning of an AU-rich element RNA-binding protein, AUF1

Crater, D, Brewer, G, DeHaven, K, Zhang, W, Schaefer, AW, DeMaria, CT, Long, L, Ehrenman, K, Wagner, BJ

Mol Cell Biol 1993
18573886 Chaperone Hsp27, a novel subunit of AUF1 protein complexes, functions in AU-rich element-mediated mRNA decay

Brewer, G, Sinsimer, KS, Krause, CD, Rivera, YM, Turrin, DK, Scrudato, S, Gupta, S, Wierzbowski, AV, Lu, J, Maher, LR, Knapinska, AM, Pestka, S, De La Cruz, MP, Gratacos, FM

Mol Cell Biol 2008
12819195 Regulation of A + U-rich element-directed mRNA turnover involving reversible phosphorylation of AUF1

Brewer, G, Wilson, GM, Huynh, Y, Sun, Y, Lu, J, Sutphen, K

J Biol Chem 2003
15257295 Concurrent versus individual binding of HuR and AUF1 to common labile target mRNAs

Gorospe, M, Yang, X, Mazan-Mamczarz, K, Lal, A, Kawai, T, Martindale, JL

EMBO J 2004
18240226 AUF1, the regulator of tumor necrosis factor alpha messenger RNA decay, is targeted by autoantibodies of patients with systemic rheumatic diseases

Smolen, J, Hueber, W, Steiner, G, Höfler, E, Skriner, K, Süleymanoglu, E, Krenn, V

Arthritis Rheum 2008
12944492 Selective degradation of AU-rich mRNAs promoted by the p37 AUF1 protein isoform

Schneider, RJ, Xi, Q, Sarkar, B, He, C

Mol Cell Biol 2003
9346902 Structural determinants in AUF1 required for high affinity binding to A + U-rich elements

Brewer, G, Sun, Y, DeMaria, CT, Wagner, BJ, Long, L

J Biol Chem 1997
11124962 Folding of A+U-rich RNA elements modulates AUF1 binding. Potential roles in regulation of mRNA turnover

Brewer, G, Chuang, Ky, Wilson, GM, Sutphen, K

J Biol Chem 2001
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