SIRT1 deacetylates HSF1

Stable Identifier
Reaction [transition]
Homo sapiens
Locations in the PathwayBrowser
SVG |   | PPTX  | SBGN
Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

Sirtuin 1 (SIRT1) functions as a NAD(+)-dependent deacetylase, which regulates the heat shock response through deacetylation of HSF1 at Lys80. The acetylation at Lys80, which is located within DNA binding domain of HSF1 disrupts HSF1 DNA-binding ability. SIRT1-mediated stress-inducible regulation of HSF1 results in prolonged HSF1 binding to the heat shock promoter of hsp70 gene. The mechanism of the enzymatic reaction which couples NAD(+) hydrolysis with lysine deacetylation in the presence of acetylated peptide is described by several groups (Landry J et al. 2000; Sauve AA et al. 2001)

Literature References
PubMed ID Title Journal Year
19914676 Substrate specificity of SIRT1-catalyzed lysine Nepsilon-deacetylation reaction probed with the side chain modified Nepsilon-acetyl-lysine analogs

Zheng, W, Pang, Y, Hirsch, BM, Jamonnak, N

Bioorg. Chem. 2010
11747420 Chemistry of gene silencing: the mechanism of NAD+-dependent deacetylation reactions

Celic, I, Avalos, J, Boeke, JD, Deng, H, Schramm, VL, Sauve, AA

Biochemistry 2001
23349863 The SIRT1 modulators AROS and DBC1 regulate HSF1 activity and the heat shock response

Mendez, JE, Brunquell, J, Nguyen, K, Westerheide, SD, Raynes, R, Pombier, KM

PLoS ONE 2013
19229036 Stress-inducible regulation of heat shock factor 1 by the deacetylase SIRT1

Sistonen, L, Morimoto, RI, Westerheide, SD, Anckar, J, Stevens, SM

Science 2009
Catalyst Activity

NAD-dependent protein deacetylase activity of SIRT1:HSP70:DNAJB1:Ac-K80-HSF1:target gene [nucleoplasm]

Orthologous Events
Cite Us!