The UBA2:SAE1 complex catalyzes the formation of a thioester linkage between the C-terminal glycine of the mature SUMO and a cysteine residue (cysteine-173) in UBA2 (SAE2) (reviewed in Wang and Dasso 2009, Wilkinson and Henley 2010, Hannoun et al. 2010, Gareau and Lima 2010). During the process the C-terminal glycine residue of SUMO is reacted with ATP to yield pyrophosphate and a transient intermediate, SUMO adenylate. The SUMO adenylate then reacts with the thiol group of the cysteine residue of UBA2 (Olsen et al. 2010).
Wang, Y, Dasso, M
Hannoun, Z, Greenhough, S, Jaffray, E, Hay, RT, Hay, DC
Olsen, SK, Capili, AD, Lu, X, Tan, DS, Lima, CD
Gareau, JR, Lima, CD
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