Reactome | SUMO is conjugated to E1 (UBA2:SAE1)

SUMO is conjugated to E1 (UBA2:SAE1)

Stable Identifier

R-HSA-3065676

Type

Pathway

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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The UBA2:SAE1 complex catalyzes the formation of a thioester linkage between the C-terminal glycine of the mature SUMO and a cysteine residue (cysteine-173) in UBA2 (SAE2) (reviewed in Wang and Dasso 2009, Wilkinson and Henley 2010, Hannoun et al. 2010, Gareau and Lima 2010). During the process the C-terminal glycine residue of SUMO is reacted with ATP to yield pyrophosphate and a transient intermediate, SUMO adenylate. The SUMO adenylate then reacts with the thiol group of the cysteine residue of UBA2 (Olsen et al. 2010).

Literature References

PubMed ID	Title	Journal	Year
20674646	Post-translational modification by SUMO Hay, RT, Hannoun, Z, Jaffray, E, Greenhough, S, Hay, DC	Toxicology	2010
19923268	SUMOylation and deSUMOylation at a glance Dasso, M, Wang, Y	J. Cell. Sci.	2009
20164921	Active site remodelling accompanies thioester bond formation in the SUMO E1 Capili, AD, Lu, X, Olsen, SK, Lima, CD, Tan, DS	Nature	2010
21102611	The SUMO pathway: emerging mechanisms that shape specificity, conjugation and recognition Gareau, JR, Lima, CD	Nat. Rev. Mol. Cell Biol.	2010