The UBA2:SAE1 complex catalyzes the formation of a thioester linkage between the C-terminal glycine of the mature SUMO and a cysteine residue (cysteine-173) in UBA2 (SAE2) (reviewed in Wang and Dasso 2009, Wilkinson and Henley 2010, Hannoun et al. 2010, Gareau and Lima 2010). During the process the C-terminal glycine residue of SUMO is reacted with ATP to yield pyrophosphate and a transient intermediate, SUMO adenylate. The SUMO adenylate then reacts with the thiol group of the cysteine residue of UBA2 (Olsen et al. 2010).
Hay, RT, Hannoun, Z, Jaffray, E, Greenhough, S, Hay, DC
Dasso, M, Wang, Y
Capili, AD, Lu, X, Olsen, SK, Lima, CD, Tan, DS
Gareau, JR, Lima, CD
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