The UBA2:SAE1 complex catalyzes the formation of a thioester bond between SUMO2 and cysteine-173 of UBA2 (Tatham et al. 2001, Werner et al. 2009). ATP reacts with the C-terminal glycine residue of SUMO2 to yield pyrophosphate and a transient intermediate, SUMO2 adenylate, which then reacts with the thiol group of the cysteine residue on UBA2.
Vaughan, OA, Tatham, MH, Botting, CH, Naismith, JH, Desterro, JM, Jaffray, E, Hay, RT
Moutty, MC, Melchior, F, Möller, U, Werner, A
SUMO activating enzyme activity of UBA2:SAE1 [nucleoplasm]
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