Conjugation of SUMO2 to UBA2:SAE1

Stable Identifier
R-HSA-2993784
Type
Reaction [transition]
Species
Homo sapiens
Compartment
nucleoplasm
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Conjugation of SUMO2 to UBA2:SAE1
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The UBA2:SAE1 complex catalyzes the formation of a thioester bond between SUMO2 and cysteine-173 of UBA2 (Tatham et al. 2001, Werner et al. 2009). ATP reacts with the C-terminal glycine residue of SUMO2 to yield pyrophosphate and a transient intermediate, SUMO2 adenylate, which then reacts with the thiol group of the cysteine residue on UBA2.

Literature References
PubMed ID Title Journal Year
19107418 Performing in vitro sumoylation reactions using recombinant enzymes

Werner, A, Moutty, MC, Möller, U, Melchior, F

Methods Mol. Biol. 2009
11451954 Polymeric chains of SUMO-2 and SUMO-3 are conjugated to protein substrates by SAE1/SAE2 and Ubc9

Tatham, MH, Jaffray, E, Vaughan, OA, Desterro, JM, Botting, CH, Naismith, JH, Hay, RT

J. Biol. Chem. 2001
Participants
Input
Output
Participant Of
hasEvent
Catalyst Activity
Catalyst Activity
Title
SUMO activating enzyme activity of UBA2:SAE1 [nucleoplasm]
Physical Entity
UBA2:SAE1 [nucleoplasm]
Activity
SUMO activating enzyme activity (0019948)
Inferred From
Conjugation of SUMO1 to UBA2:SAE1 (Homo sapiens)
Authored
May, B  (2013-01-16)
Reviewed
Garg, AK  (2013-05-16)
Created
May, B  (2013-01-19)