The UBA2:SAE1 complex catalyzes the formation of a thioester bond between SUMO1 and cysteine-173 of UBA2 (Desterro et al. 1999, Okuma et al. 1999, Werner et al. 2009, Olsen et al. 2010, Wang and Chen 2010). ATP reacts with the C-terminal glycine residue of SUMO1 to yield pyrophosphate and a transient intermediate, SUMO1 adenylate, which then reacts with the thiol group of the cysteine residue on UBA2.
Moutty, MC, Melchior, F, Möller, U, Werner, A
Desterro, JM, Kemp, GD, Rodriguez, MS, Hay, RT
Wang, J, Chen, Y
Capili, AD, Lu, X, Olsen, SK, Lima, CD, Tan, DS
Yasuda, H, Honda, R, Tsumagari, N, Ichikawa, G, Okuma, T
SUMO activating enzyme activity of UBA2:SAE1 [nucleoplasm]
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