The UBA2:SAE1 complex catalyzes the formation of a thioester bond between SUMO1 and cysteine-173 of UBA2 (Desterro et al. 1999, Okuma et al. 1999, Werner et al. 2009, Olsen et al. 2010, Wang and Chen 2010). ATP reacts with the C-terminal glycine residue of SUMO1 to yield pyrophosphate and a transient intermediate, SUMO1 adenylate, which then reacts with the thiol group of the cysteine residue on UBA2.
Desterro, JM, Rodriguez, MS, Kemp, GD, Hay, RT
Okuma, T, Honda, R, Ichikawa, G, Tsumagari, N, Yasuda, H
Werner, A, Moutty, MC, Möller, U, Melchior, F
Olsen, SK, Capili, AD, Lu, X, Tan, DS, Lima, CD
Wang, J, Chen, Y
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