Reactome | Conjugation of SUMO1 to UBA2:SAE1

Conjugation of SUMO1 to UBA2:SAE1

Stable Identifier

R-HSA-2990833

Type

Reaction [transition]

Species

Homo sapiens

Compartment

nucleoplasm

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The UBA2:SAE1 complex catalyzes the formation of a thioester bond between SUMO1 and cysteine-173 of UBA2 (Desterro et al. 1999, Okuma et al. 1999, Werner et al. 2009, Olsen et al. 2010, Wang and Chen 2010). ATP reacts with the C-terminal glycine residue of SUMO1 to yield pyrophosphate and a transient intermediate, SUMO1 adenylate, which then reacts with the thiol group of the cysteine residue on UBA2.

Literature References

PubMed ID	Title	Journal	Year
10187858	Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1 Desterro, JM, Rodriguez, MS, Kemp, GD, Hay, RT	J. Biol. Chem.	1999
9920803	In vitro SUMO-1 modification requires two enzymatic steps, E1 and E2 Okuma, T, Honda, R, Ichikawa, G, Tsumagari, N, Yasuda, H	Biochem. Biophys. Res. Commun.	1999
19107418	Performing in vitro sumoylation reactions using recombinant enzymes Werner, A, Moutty, MC, Möller, U, Melchior, F	Methods Mol. Biol.	2009
20164921	Active site remodelling accompanies thioester bond formation in the SUMO E1 Olsen, SK, Capili, AD, Lu, X, Tan, DS, Lima, CD	Nature	2010
20501649	Role of the Zn(2+) motif of E1 in SUMO adenylation Wang, J, Chen, Y	J. Biol. Chem.	2010