TGFBR1 is recruited to tight junctions by PARD6A

Stable Identifier
R-HSA-2134506
Type
Reaction [binding]
Species
Homo sapiens
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TGFBR1 binds to PARD6A, a component of tight junctions, and localizes to tight junctions irrespective of TGF-beta stimulation. The N-terminus of PARD6A, containing a PB1 domain necessary for interactions with PRKCZ, is necessary for binding to TGFBR1 (Ozdamar et al. 2005).
PARD6A, bound to PARD3 and PRKCZ, is associated with tight junctions through JAM-A (Ebnet et al. 2001), which is bound to CGN (cingulin) (Bazzoni et al. 2000). CGN binds ARHGEF18 (p114RhoGEF), and ARHGEF18 recruits RHOA to tight junctions. Other components of the tight junction structure are not shown in this context (Terry et al. 2011).
Junctional RHOA activity is required for maintenance of junctional integrity through regulation of actinomyosin cytoskeleton organization (Terry et al. 2011). This was inferred from experiments in which a recombinant mouse Pard6a and recombinant human TGFBR1 were studied in the context of endogenous mouse tight junctions.

Literature References
PubMed ID Title Journal Year
10877843 Interaction of junctional adhesion molecule with the tight junction components ZO-1, cingulin, and occludin

Bazzoni, G, Martinez-Estrada, OM, Orsenigo, F, Cordenonsi, M, Citi, S, Dejana, E

J Biol Chem 2000
15761148 Regulation of the polarity protein Par6 by TGFbeta receptors controls epithelial cell plasticity

Ozdamar, B, Bose, R, Barrios-Rodiles, M, Wang, HR, Zhang, Y, Wrana, JL

Science 2005
21258369 Spatially restricted activation of RhoA signalling at epithelial junctions by p114RhoGEF drives junction formation and morphogenesis

Terry, SJ, Zihni, C, Elbediwy, A, Vitiello, E, Leefa Chong San, IV, Balda, MS, Matter, K

Nat Cell Biol 2011
12953056 The junctional adhesion molecule (JAM) family members JAM-2 and JAM-3 associate with the cell polarity protein PAR-3: a possible role for JAMs in endothelial cell polarity

Ebnet, K, Aurrand-Lions, M, Kuhn, A, Kiefer, F, Butz, S, Zander, K, Meyer zu Brickwedde, MK, Suzuki, A, Imhof, BA, Vestweber, D

J Cell Sci 2003
11447115 The cell polarity protein ASIP/PAR-3 directly associates with junctional adhesion molecule (JAM)

Ebnet, K, Suzuki, A, Horikoshi, Y, Hirose, T, Meyer zu Brickwedde, MK, Ohno, S, Vestweber, D

EMBO J 2001
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