CYP27B1 hydroxylates 25(OH)D to 1,25(OH)2D

Stable Identifier
Reaction [transition]
Homo sapiens
Locations in the PathwayBrowser
SVG |   | PPTX  | SBGN
Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

The second step in vitamin D activation requires hydroxylation of 25-hydroxyvitamin D (calcidiol, 25(OH)D) to 1-alpha, 25-dihydroxyvitamin D (1,25(OH)2D). This conversion is mediated by 25-hydroxyvitamin D-1-alpha hydroxylase (CYP27B1), an outer mitochondrial membrane-resident protein (Zehnder et al. 2002, Fritsche et al. 2003, Sawada et al. 1999).

Literature References
PubMed ID Title Journal Year
11856765 Synthesis of 1,25-dihydroxyvitamin D(3) by human endothelial cells is regulated by inflammatory cytokines: a novel autocrine determinant of vascular cell adhesion

Zehnder, D, Stewart, PM, Howie, AJ, Bland, R, Wheeler, DC, Hewison, M, Williams, MC, Chana, RS

J Am Soc Nephrol 2002
12855575 Regulation of 25-hydroxyvitamin D3-1 alpha-hydroxylase and production of 1 alpha,25-dihydroxyvitamin D3 by human dendritic cells

Kreutz, M, Andreesen, R, Mondal, K, Fritsche, J, Ehrnsperger, A

Blood 2003
10518789 Enzymatic properties of human 25-hydroxyvitamin D3 1alpha-hydroxylase coexpression with adrenodoxin and NADPH-adrenodoxin reductase in Escherichia coli

Kato, S, Sakaki, T, Sawada, N, Inouye, K, Takeyama, K, Kitanaka, S

Eur. J. Biochem. 1999
Catalyst Activity

calcidiol 1-monooxygenase activity of CYP27B1(?-508) [mitochondrial outer membrane]

This event is regulated
Negatively by
Orthologous Events
Cite Us!