At higher concentrations of ligand, a substantial fraction of the receptor (>50%) is endocytosed through a clathrin independent, lipid-raft-dependent route as the receptor becomes Y1045 phosphorylated and ubiquitnated. Eps15 and Epsin are found in caveolae. Eps15 and Epsin are immunoprecipated with the EGF receptor. Non-clathrin internalization of ubiquitinated EGFR depends on its interaction with proteins harbouring the UIM Ub-interacting motif, as shown through the ablation of three Ub-interacting motif-containing proteins, Eps15, Eps15R and Epsin.
Sigismund, S, Woelk, T, Puri, C, Maspero, E, Tacchetti, C, Transidico, P, Di Fiore, PP, Polo, S
Klapisz, E, Sorokina, I, Lemeer, S, Pijnenburg, M, Verkleij, AJ, van Bergen en Henegouwen, PM
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