At higher concentrations of ligand, a substantial fraction of the receptor (>50%) is endocytosed through a clathrin independent, lipid-raft-dependent route as the receptor becomes Y1045 phosphorylated and ubiquitnated. Eps15 and Epsin are found in caveolae. Eps15 and Epsin are immunoprecipated with the EGF receptor. Non-clathrin internalization of ubiquitinated EGFR depends on its interaction with proteins harbouring the UIM Ub-interacting motif, as shown through the ablation of three Ub-interacting motif-containing proteins, Eps15, Eps15R and Epsin.
Pijnenburg, M, Lemeer, S, van Bergen en Henegouwen, PM, Sorokina, I, Klapisz, E, Verkleij, AJ
Di Fiore, PP, Tacchetti, C, Transidico, P, Puri, C, Maspero, E, Polo, S, Sigismund, S, Woelk, T
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