Removal of remaining Flap from the C-strand

Stable Identifier
R-HSA-174446
Type
Reaction [transition]
Species
Homo sapiens
Compartment
nucleoplasm
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Removal of remaining Flap from the C-strand
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The remaining flap, which is too short to support RPA binding, is then processed by FEN-1. There is evidence that binding of RPA to the displaced end of the RNA-containing Okazaki fragment prevents FEN-1 from accessing the substrate. FEN-1 is a structure-specific endonuclease that cleaves near the base of the flap at a position one nucleotide into the annealed region. Biochemical studies have shown that the preferred substrate for FEN-1 consists of a one-nucleotide 3'-tail on the upstream primer in addition to the 5'-flap of the downstream primer.

Participants
Input
Output
Participant Of
hasEvent
Event Information
Go Biological Process
telomere maintenance via semi-conservative replication (0032201)
Catalyst Activity
Catalyst Activity
Title
5'-flap endonuclease activity of FEN1 [nucleoplasm]
Physical Entity
FEN1 [nucleoplasm]
Activity
5'-flap endonuclease activity (0017108)
Orthologous Events
Removal of remaining Flap from the C-strand (Bos taurus)
Removal of remaining Flap from the C-strand (Caenorhabditis elegans)
Removal of remaining Flap from the C-strand (Canis familiaris)
Removal of remaining Flap from the C-strand (Danio rerio)
Removal of remaining Flap from the C-strand (Dictyostelium discoideum)
Removal of remaining Flap from the C-strand (Mus musculus)
Removal of remaining Flap from the C-strand (Rattus norvegicus)
Removal of remaining Flap from the C-strand (Schizosaccharomyces pombe)
Removal of remaining Flap from the C-strand (Sus scrofa)
Removal of remaining Flap from the C-strand (Xenopus tropicalis)
Authored
Blackburn, EH  (2006-03-10)
Seidel, J  (2006-03-10)
Reviewed
Price, C  (2006-07-13)
Created
Gillespie, ME  (2006-02-18)