Phosphorylation and release of IRF3

Stable Identifier
R-HSA-1606327
Type
Reaction [dissociation]
Species
Homo sapiens
Related Species
Human cytomegalovirus, Human alphaherpesvirus 2
Compartment
cytosol
Locations in the PathwayBrowser
Expand all
General
SVG | 
PNG
Low
Medium
High
 | PPTX  | SBGN
Phosphorylation and release of IRF3
Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

IRF3 is activated through a two-step phosphorylation in the C-terminal domain mediated by TBK1 and/or IKKi, requiring Ser386 and/or Ser385- site 1; and a cluster of serine/threonine residues between Ser396 and Ser405- site 2 [Panne et al 2007]. Phosphorylated residues at site 2 (Ser396 - Ser405) alleviate autoinhibition to allow interaction with CBP (CREB-binding protein) and facilitate phosphorylation at site 1 (Ser385 or Ser386). Phosphorylation at site 1 is required for IRF3 dimerization.

Literature References
PubMed ID Title Journal Year
17526488 Interferon regulatory factor 3 is regulated by a dual phosphorylation-dependent switch

Panne, D, McWhirter, SM, Maniatis, T, Harrison, SC

J Biol Chem 2007
12692549 IKKepsilon and TBK1 are essential components of the IRF3 signaling pathway

Fitzgerald, KA, McWhirter, SM, Faia, KL, Rowe, DC, Latz, E, Golenbock, DT, Coyle, AJ, Liao, SM, Maniatis, T

Nat Immunol 2003
9463386 Direct triggering of the type I interferon system by virus infection: activation of a transcription factor complex containing IRF-3 and CBP/p300

Yoneyama, M, Suhara, W, Fukuhara, Y, Fukuda, M, Nishida, E, Fujita, T

EMBO J 1998
Participants
Input
Output
Participant Of
hasEvent
This event is regulated
Negatively by
Authored
Shamovsky, V  (2011-09-21)
Reviewed
D'Eustachio, P  (2011-12-08)
Upton, JW  (2012-02-19)
Mocarski, ES  (2012-02-19)
Created
Shamovsky, V  (2011-09-21)