TMP, (d)UMP, uridine 2' monophosphate, or uridine 3'-monophosphate + H2O => thymidine, deoxyuridine, or uridine + orthophosphate [NT5M]

Stable Identifier
R-HSA-109514
Type
Reaction [transition]
Species
Homo sapiens
Compartment
mitochondrial matrix
ReviewStatus
5/5
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TMP, (d)UMP, uridine 2' monophosphate, or uridine 3'-monophosphate + H2O => thymidine, deoxyuridine, or uridine + orthophosphate [NT5M]
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5'3'-Nucleotidase, mitochondrial (NT5M) is the major nucleotidase of human mitochondria, catalyzing the hydrolysis of TMP, uridine 2'-, 3'-, and 5'-monophosphates, and dUMP to yield the corresponding (deoxy)nucleosides and orthophosphate. It may play a central role in "substrate cycles" to regulate mitochondrial deoxynucleotide levels, especially in non-dividing cells (Rampazzo et al. 2000; Gallinaro et al. 2002). The active form of the enzyme is a homodimer, with an absolute requirement for Mg++ (Rampazzo et al. 2000; Rinaldo-Matthis et al. 2002).
Literature References
PubMed ID Title Journal Year
12124385 Human mitochondrial 5'-deoxynucleotidase. Overproduction in cultured cells and functional aspects

Gallinaro, L, Crovatto, K, Rampazzo, C, Pontarin, G, Ferraro, P, Milanese, E, Reichard, PA, Bianchi, V

J Biol Chem 2002
12352955 Crystal structure of a human mitochondrial deoxyribonuclease

Rinaldo-Matthis, A, Rampazzo, C, Reichard, PA, Bianchi, V, Nordlund, P

Nt Struct Biol 2002
10899995 A deoxynucleotidase in mitochondria: involvement in regulation of dNTP pools and possible link to genetic disease

Rampazzo, C, Gallinaro, L, Milanese, E, Frigimelica, E, Reichard, PA, Bianchi, V

Proc Natl Acad Sci USA 2000
Participants
Input
Output
Participates
as an event of
Catalyst Activity

5'-nucleotidase activity of NT5M dimer [mitochondrial matrix]

Physical Entity
NT5M dimer [mitochondrial matrix] (Homo sapiens)
Activity
5'-nucleotidase activity (GO:0008253)
Orthologous Events
TMP, (d)UMP, uridine 2' monophosphate, or uridine 3'-monophosphate + H2O => thymidine, deoxyuridine, or uridine + orthophosphate [NT5M] (Bos taurus)
TMP, (d)UMP, uridine 2' monophosphate, or uridine 3'-monophosphate + H2O => thymidine, deoxyuridine, or uridine + orthophosphate [NT5M] (Canis familiaris)
TMP, (d)UMP, uridine 2' monophosphate, or uridine 3'-monophosphate + H2O => thymidine, deoxyuridine, or uridine + orthophosphate [NT5M] (Gallus gallus)
TMP, (d)UMP, uridine 2' monophosphate, or uridine 3'-monophosphate + H2O => thymidine, deoxyuridine, or uridine + orthophosphate [NT5M] (Mus musculus)
TMP, (d)UMP, uridine 2' monophosphate, or uridine 3'-monophosphate + H2O => thymidine, deoxyuridine, or uridine + orthophosphate [NT5M] (Rattus norvegicus)
TMP, (d)UMP, uridine 2' monophosphate, or uridine 3'-monophosphate + H2O => thymidine, deoxyuridine, or uridine + orthophosphate [NT5M] (Sus scrofa)
TMP, (d)UMP, uridine 2' monophosphate, or uridine 3'-monophosphate + H2O => thymidine, deoxyuridine, or uridine + orthophosphate [NT5M] (Xenopus tropicalis)
Created
D'Eustachio, P  (2004-01-09)
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