Reactome | (d)CMP, TMP, or (d)UMP + H2O => (deoxy)cytidine, thymidine, or (deoxy)uridine + orthophosphate (NT5C3)

(d)CMP, TMP, or (d)UMP + H2O => (deoxy)cytidine, thymidine, or (deoxy)uridine + orthophosphate (NT5C3)

Stable Identifier

R-HSA-109449

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol

ReviewStatus

5/5

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(d)CMP, TMP, or (d)UMP + H2O => (deoxy)cytidine, thymidine, or (deoxy)uridine + orthophosphate (NT5C3)

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Cytosolic 5'-nucleotidase 3 (NT5C3) catalyzes the hydrolysis of pyrimidine nucleoside monophosphates (d)CMP, TMP, and (d)UMP to nucleosides plus orthophosphate. While the enzyme appears to be present in many tissues, it is especially abundant in erythrocytes, where it may function to remove excess pyrimidine nucleotides generated by nucleic acid breakdown, while sparing purine nucleotides needed for red cell energy metabolism. Deficiencies in the enzyme are associated with a form of hemolytic anemia and its inactivation by heavy metals may be responsible for some hematological abnormalities associated with lead poisoning (Marinaki et al. 2001; Rees et al. 2003). The active form of the enzyme is a monomer. It has an absolute requirement for Mg++, and is inactive against purine nucleotides (Amici et al. 1997; Amici and Magni 2002).

Literature References

PubMed ID	Title	Journal	Year
9428647	Pyrimidine nucleotidases from human erythrocyte possess phosphotransferase activities specific for pyrimidine nucleotides Raffaelli, N, Ruggieri, S, Emmanuelli, M, Magni, G, Amici, A	FEBS Lett	1997
12580951	Pyrimidine 5' nucleotidase deficiency Marinaki, AM, Duley, JA, Rees, DC	Br J Haematol	2003
11369620	Genetic basis of hemolytic anemia caused by pyrimidine 5' nucleotidase deficiency Seip, M, Magni, G, Naponelli, V, Amici, A, Simmonds, A, Marinaki, AM, Duley, JA, Escuredo, E, Thein, SL, Rees, DC, Ben-Bassat, I, Harley, EH	Blood	2001
11795870	Human erythrocyte pyrimidine 5'-nucleotidase, PN-I Magni, G, Amici, A	Arch Biochem Biophys	2002