(d)CMP, TMP, or (d)UMP + H2O => (deoxy)cytidine, thymidine, or (deoxy)uridine + orthophosphate (NT5C3)

Stable Identifier
Reaction [transition]
Homo sapiens
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Cytosolic 5'-nucleotidase 3 (NT5C3) catalyzes the hydrolysis of pyrimidine nucleoside monophosphates (d)CMP, TMP, and (d)UMP to nucleosides plus orthophosphate. While the enzyme appears to be present in many tissues, it is especially abundant in erythrocytes, where it may function to remove excess pyrimidine nucleotides generated by nucleic acid breakdown, while sparing purine nucleotides needed for red cell energy metabolism. Deficiencies in the enzyme are associated with a form of hemolytic anemia and its inactivation by heavy metals may be responsible for some hematological abnormalities associated with lead poisoning (Marinaki et al. 2001; Rees et al. 2003). The active form of the enzyme is a monomer. It has an absolute requirement for Mg++, and is inactive against purine nucleotides (Amici et al. 1997; Amici and Magni 2002).

Literature References
PubMed ID Title Journal Year
9428647 Pyrimidine nucleotidases from human erythrocyte possess phosphotransferase activities specific for pyrimidine nucleotides

Raffaelli, N, Ruggieri, S, Emmanuelli, M, Magni, G, Amici, A

FEBS Lett 1997
12580951 Pyrimidine 5' nucleotidase deficiency

Marinaki, AM, Duley, JA, Rees, DC

Br J Haematol 2003
11369620 Genetic basis of hemolytic anemia caused by pyrimidine 5' nucleotidase deficiency

Seip, M, Magni, G, Naponelli, V, Amici, A, Simmonds, A, Marinaki, AM, Duley, JA, Escuredo, E, Thein, SL, Rees, DC, Ben-Bassat, I, Harley, EH

Blood 2001
11795870 Human erythrocyte pyrimidine 5'-nucleotidase, PN-I

Magni, G, Amici, A

Arch Biochem Biophys 2002
Catalyst Activity

5'-nucleotidase activity of NT5C3 holoenzyme [cytosol]

Orthologous Events
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