UniProt:P37653 bcsA

chain
  • chain:1-872
checksum 14326B8A2EB228F7
comment
  • FUNCTION Catalytic subunit of cellulose synthase. It polymerizes uridine 5'-diphosphate glucose to cellulose, which is produced as an extracellular component for mechanical and chemical protection at the onset of the stationary phase, when the cells exhibit multicellular behavior (rdar morphotype). Coexpression of cellulose and thin aggregative fimbriae (curli fimbrae or fibers) leads to a hydrophobic network with tightly packed cells embedded in a highly inert matrix that confers cohesion, elasticity and tissue-like properties to colonies (PubMed:24097954).CATALYTIC ACTIVITY [(1->4)-beta-D-glucosyl](n) + UDP-alpha-D-glucose = [(1->4)-beta-D-glucosyl](n+1) + UDP + H(+)COFACTOR Activated by bis-(3'-5') cyclic diguanylic acid (c-di-GMP).PATHWAY Glycan metabolism; bacterial cellulose biosynthesis.SUBCELLULAR LOCATION Part of the yhjR-bcsQABZC operon. Expressed at low levels in mid-log phase, expression increases as cells enter stationary phase, the increase in stationary phase is dependent on rpoS (PubMed:24097954). Expression is higher at 28 than 37 degrees Celsius (at protein level) (PubMed:24097954).DOMAIN There are two conserved domains in the globular part of the protein: the N-terminal domain (domain A) contains the conserved DXD motif and is possibly involved in catalysis and substrate binding. The C-terminal domain (domain B) contains the QXXRW motif and is present only in processive glycosyl transferases. It could be involved in the processivity function of the enzyme, possibly required for holding the growing glycan chain in the active site.DISRUPTION PHENOTYPE When disrupted in a cellulose-synthesizing strain (a strain K12 / W3110 derivative called AR3110 with a restored, functional bcsQ gene), cellulose is no longer made and the rdar morphotype is lost (PubMed:24097954).MISCELLANEOUS The genes bscA, bcsB, bcsZ and bcsC are constitutively transcribed but cellulose synthesis occurs only when DgcC, a putative transmembrane protein regulated by CsgD, is expressed. Cellulose production is abolished in E.coli K12 / MG1655 and W3110 due to a premature stop codon in bcsQ (PubMed:24097954).SIMILARITY Belongs to the glycosyltransferase 2 family.SEQUENCE CAUTION Extended N-terminus.
created [InstanceEdit:9932217] Stephan, Ralf, 2024-12-18
crossReference
databaseName UniProt
dbId 9932203
description
  • recommendedName: Cellulose synthase catalytic subunit [UDP-forming] ecNumber: 2.4.1.12
displayName UniProt:P37653 bcsA
geneName
  • bcsA
  • yhjO
  • yhjP
  • b3533
  • JW5665
identifier P37653
isSequenceChanged false
keyword
  • 3D-structure
  • c-di-GMP
  • Cell inner membrane
  • Cell membrane
  • Cellulose biosynthesis
  • Glycosyltransferase
  • Membrane
  • Reference proteome
  • Transferase
  • Transmembrane
  • Transmembrane helix
modified [InstanceEdit:9939033] Weiser, Joel, 2025-02-21
moleculeType Protein
name
  • bcsA
physicalEntity
referenceDatabase [ReferenceDatabase:2] UniProt
referenceGene
referenceTranscript
schemaClass ReferenceGeneProduct
secondaryIdentifier
  • BCSA_ECOLI
  • P37654
  • P76712
  • P76713
  • Q2M7J5
  • Q8RSS7
sequenceLength 872
species [Species:159879] Escherichia coli
stId uniprot:P37653
url http://purl.uniprot.org/uniprot/P37653
Cite Us!