Reactome | glutamine + H2O => glutamate + NH4+ (GLS)

Schema > Reaction > Entries

glutamine + H2O => glutamate + NH4+ (GLS) Show undefined attributes

catalystActivity	[CatalystActivity:10652847] glutaminase activity of GLS dimers [mitochondrial matrix]
category	transition
compartment	[Compartment:5460] mitochondrial matrix
created	[InstanceEdit:10651445] Weiser, Joel, 2026-03-16
dbId	10652849
displayName	glutamine + H2O => glutamate + NH4+ (GLS)
eventOf	[Pathway:R-XTR-210500] Glutamate Neurotransmitter Release Cycle - Xenopus tropicalis [Pathway:R-XTR-8964539] Glutamate and glutamine metabolism - Xenopus tropicalis [Pathway:R-XTR-5628897] TP53 Regulates Metabolic Genes - Xenopus tropicalis
evidenceType	[EvidenceType:9984902] inferred by electronic annotation
followingEvent	[Reaction:R-XTR-508040] glutamate + ATP + NADPH + H+ => L-glutamate gamma-semialdehyde + NADP+ + ADP + orthophosphate [P5CS] - Xenopus tropicalis [Reaction:R-XTR-70666] glutamate + L-glutamate gamma-semialdehyde <=> ornithine + alpha-ketoglutarate [OAT] - Xenopus tropicalis [Reaction:R-XTR-70600] glutamate + NAD(P)+ => alpha-ketoglutarate + NH4+ + NAD(P)H + H+ (GLUD1,2) - Xenopus tropicalis [Reaction:R-XTR-210444] L-Glutamate loading of synaptic vesicle - Xenopus tropicalis
inferredFrom	[Reaction:R-HSA-70609] glutamine + H2O => glutamate + NH4+ (GLS) - Homo sapiens
input	[SimpleEntity:R-ALL-113521] H2O [mitochondrial matrix] [SimpleEntity:R-ALL-113522] L-Gln [mitochondrial matrix]
isInDisease	false
isInferred	true
maxDepth	1
name	glutamine + H2O => glutamate + NH4+ (GLS) H2O + L-glutamine => L-glutamate + NH4(+)
output	[SimpleEntity:R-ALL-113552] L-Glu [mitochondrial matrix] [SimpleEntity:R-ALL-113561] NH4+ [mitochondrial matrix]
precedingEvent	[Reaction:R-XTR-212614] Glutamine transport from astrocytes - Xenopus tropicalis
releaseDate	2026-03-25
schemaClass	Reaction
species	[Species:205621] Xenopus tropicalis
stId	R-XTR-70609
summation	[Summation:9984901] This event has been computationally inferred from an event that has been demonstrated in another species. The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation. More details and caveats of the event inference in Reactome. For details on PANTHER see also: http://www.pantherdb.org/about.jsp

Referrals

(hasEvent)	[Pathway:R-XTR-5628897] TP53 Regulates Metabolic Genes [Pathway:R-XTR-8964539] Glutamate and glutamine metabolism [Pathway:R-XTR-210500] Glutamate Neurotransmitter Release Cycle
(precedingEvent)	[Reaction:R-XTR-210444] L-Glutamate loading of synaptic vesicle [Reaction:R-XTR-508040] glutamate + ATP + NADPH + H+ => L-glutamate gamma-semialdehyde + NADP+ + ADP + orthophosphate [P5CS] [Reaction:R-XTR-70666] glutamate + L-glutamate gamma-semialdehyde <=> ornithine + alpha-ketoglutarate [OAT] [Reaction:R-XTR-70600] glutamate + NAD(P)+ => alpha-ketoglutarate + NH4+ + NAD(P)H + H+ (GLUD1,2)
(inferredTo)	[Reaction:R-HSA-70609] glutamine + H2O => glutamate + NH4+ (GLS)