Biochemical and affinity purification steps performed on mou...

created [InstanceEdit:9934136] Rothfels, Karen, 2025-01-06
dbId 9934135
displayName Biochemical and affinity purification steps performed on mou...
modified [InstanceEdit:9934292] Rothfels, Karen, 2025-01-07
schemaClass Summation
text Biochemical and affinity purification steps performed on mouse ES cells, mouse embryonic fibroblasts and newborn mouse brain extracts identified two distinct BAF complexes governing neuronal differentiation (Olave et al, 2002; Lessard et al, 2007; Staahl et al, 2013). As neuronal progenitors (np) differentiate and exit the mitotic cell cycle, the components of the npBAF complex alter. npBAF-specific subunits ACTL6A, SS18 and DPF2/PHF10 are replaced by neuron-specific subunits ACTL6B, SS18L1 and DPF1 or DPF3 (Olave et al, 2002; Lessard et al, 2007; Staahl et al, 2013; reviewed in Staahl and Crabtree, 2013; Kadoch and Crabtree, 2015; Innis and Cabot, 2020; Cenik and Shilatifard, 2021). PHF10/BAF45A and ACTL6A/BAF53A are required for proliferation of neural stem cells, and extending the expression of these subunits disrupts neuronal differentiation (Lessard et al, 2007).
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