Reactome | Discovery of an RNA virus 3'->5' exoribonuclease that is critically involved in coronavirus RNA synthesis

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Discovery of an RNA virus 3'->5' exoribonuclease that is critically involved in coronavirus RNA synthesis Show undefined attributes

author	[Person:9682559] Minskaia, E [Person:9682558] Hertzig, T [Person:9614522] Gorbalenya, AE [Person:9682556] Campanacci, V [Person:9682561] Cambillau, C [Person:9681646] Canard, B [Person:9614535] Ziebuhr, J
created	[InstanceEdit:9682557] Orlic-Milacic, Marija, 2020-04-09
dbId	9682560
displayName	Discovery of an RNA virus 3'->5' exoribonuclease that is critically involved in coronavirus RNA synthesis
journal	Proc. Natl. Acad. Sci. U.S.A.
pages	5108-13
pubMedIdentifier	16549795
schemaClass	LiteratureReference
title	Discovery of an RNA virus 3'->5' exoribonuclease that is critically involved in coronavirus RNA synthesis
url	http://www.ncbi.nlm.nih.gov/pubmed/16549795
volume	103
year	2006

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[Summation:9694297] This COVID-19 event has been created by a combination of computational inference (see https://reactome.org/documentation/inferred-events) from SARS-CoV-1 data and manual curation, as described in the summation for the overall SARS-CoV-2 infection pathway.

Non-structural protein 14 (nsp14) of the human SARS coronavirus is a bifunctional enzyme bearing 3'-5 exoribonuclease activity involved in replication fidelity and RNA cap N7-guanine methyltransferase activity involved in 5'-RNA capping. nsp14 binds to the minimal replication and transcription complex (RTC), composed of nsp7, nsp8, and nsp12, by directly binding to nsp12 (the main RNA-dependent RNA polymerase). Binding of nsp14 does not affect the processivity of the RTC (Minskaia et al. 2006, Subissi et al. 2014).
[Reaction:R-HSA-9682258] nsp14 binds nsp12
[Reaction:R-HSA-9682603] nsp14 acts as a 3'-to-5' exonuclease to remove misincorporated nucleotides from nascent RNA
[Summation:9694564] In human SARS coronavirus 2 (SARS-CoV-2) nsp14 is essential ...

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