Virally encoded RNA-dependent RNA polymerase (nsp12, also kn...

created [InstanceEdit:9681829] Orlic-Milacic, Marija, 2020-04-06
dbId 9681828
displayName Virally encoded RNA-dependent RNA polymerase (nsp12, also kn...
modified [InstanceEdit:9687591] Orlic-Milacic, Marija, 2020-05-11
schemaClass Summation
text Virally encoded RNA-dependent RNA polymerase (nsp12, also known as RdRP) is the key component of the replication transcription complex (RTC). As the human SARS coronavirus 1 (SARS-CoV-1) is a plus strand RNA virus, nsp12 first synthesizes the complementary minus RNA strand. The purified SARS-CoV-1 nsp12 shows both primer dependent and primer-independent RNA synthesis activities using homopolymeric RNA templates. The catalytic activity of nsp12 is strictly dependent on manganese ions (Mn2+) and primers when the template is a viral-genome-derived RNA representing part of the 3?-UTR of the plus strand with a polyA tail. A 36 nucleotide sequence from the 3?-UTR, predicted to form a stable stem-loop structure, seems to be the minimal cis-acting RNA element required for nsp12 to initiate RNA synthesis (Ahn et al. 2012). The complex of nsp7 and nsp8 confers processivity to nsp12 (Subissi et al. 2014).
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