The DNA ligase complex composed of DNA ligase 4 (LIG4) and t...

created [InstanceEdit:5693618] Orlic-Milacic, Marija, 2015-05-16
dbId 5693541
displayName The DNA ligase complex composed of DNA ligase 4 (LIG4) and t...
modified [InstanceEdit:6784748] Orlic-Milacic, Marija, 2015-06-22
schemaClass Summation
text The DNA ligase complex composed of DNA ligase 4 (LIG4) and the XRCC4 homodimer (Sibanda et al. 2000) catalyzes ligation of DNA double strand break (DSB) ends during non-homologous end joining (NHEJ). The XRCC4:LIG4 complex is recruited to NHEJ sites through interaction of its subunits with XRCC5:XRCC6 (Ku complex), PRKDC (DNA-PKcs) and DCLRE1C (ARTEMIS) (McElhinny et al. 2000, Hsu et al. 2002, Malu et al. 2012). Phosphorylation of XRCC4 by PRKDC may regulate the activity of the ligase complex (Lee et al. 2004). XRCC4:LIG4 can ligate incompatible DNA DSB ends, and may also ligate across single nucleotide gaps (Gu et al. 2007). The presence of the accessory protein NHEJ1 (XLF) facilitates XRCC4:LIG4 ligase activity, especially at mismatched DNA DSB ends (Ahnesorg et al. 2006, Buck et al. 2006, Tsai et al. 2007). Depending on other types of DNA damage present at DNA DSBs, NHEJ can result in error-free products, produce dsDNA with microdeletions and/or mismatched bases, or result in translocations (reviewed by Povirk 2012).
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