Tumor necrosis factor ? (TNF-?)-induced apoptosis is regulat...

created [InstanceEdit:179407] Gopinathrao, G, 2006-04-27 13:13:48
dbId 179240
displayName Tumor necrosis factor ? (TNF-?)-induced apoptosis is regulat...
literatureReference
modified [InstanceEdit:9828914] Shamovsky, Veronica, 2023-02-20
schemaClass Summation
text Tumor necrosis factor ? (TNF-?)-induced apoptosis is regulated by the activity of caspase-8 (CASP8) (Micheau O and Tschopp J 2003; Justus SJ and Ting AT 2015). CASP8 is synthesized as an inactive precursor, pro-caspase-8, which is recruited to the cytosolic TRADD:TRAF2:RIPK1:FADD complex upon stimulation. Within this complex, multiple procaspase?8 molecules interact via their tandem death?effector domains (DED), thereby facilitating both proximity?induced dimerization and proteolytic cleavage of procaspase?8, which are required for initiation of apoptotic cell death (Keller N et al 2009; Oberst A et al. 2010). Activated CASP8 mediates cleavage and activation of effector caspases such as CASP3 to promote apoptosis, while cleavage of RIPK1, RIPK3 by CASP8 prevents RIPK1:RIPK3-dependent necroptosis (Parrish AB et al. 2013; Hopkins-Donaldson S et al. 2000; Newton K et al. 2019; Lalaoui N et al. 2020). When apoptosis is blocked, which occurs naturally in some cells or under certain pathophysiological conditions (e.g., expression of CASP8 inhibitory proteins such as CrmA and vICA after infection with cowpox virus or CMV and/or protein synthesis blockade that prevents NF-kappa-B-mediated expression of FLICE-inhibitory protein (cellular FLIP, CFLAR), deubiquitinated RIPK1 interacts with RIPK3 and MLKL to trigger necroptosis (Ofengeim D et al. 2015; Yuan J et al. 2019).

This Reactome event shows pro-CASP8 binding to the cytosolic TRADD:TRAF2:RIPK1:FADD complex. This binding event is negatively regulated by optineurin (OPTN) (Nakazawa S et al. 2016).
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