CDT1 binds MCM2-7 complex in budding yeast

Stable Identifier
R-SCE-9749300
Type
Reaction [binding]
Species
Saccharomyces cerevisiae
Compartment
nucleoplasm
ReviewStatus
5/5
General
SVG | 
PNG
Low
Medium
High
 | PPTX  | SBGN
CDT1 binds MCM2-7 complex in budding yeast
Based on studies in budding yeast, CDT1 binds to the MCM2-7 complex by interacting with its MCM2 and MCM6 subunits (Kawasaki et al. 2006; Remus et al. 2009), resulting in the formation of a hetero-heptameric CDT1:MCM2-7 complex (Sun et al. 2013). CDT1 binding relieves the autoinhibitory action of the MCM6 subunit that prevents association of the MCM2-7 complex with the complex of ORC and CDC6 in the absence of CDT1 (Fernández-Cid et al. 2013, Sun et al. 2013).
Literature References
PubMed ID Title Journal Year
16824194 Reconstitution of Saccharomyces cerevisiae prereplicative complex assembly in vitro

Kawasaki, Y, Kim, HD, Kojima, A, Seki, T, Sugino, A

Genes Cells 2006
19896182 Concerted loading of Mcm2-7 double hexamers around DNA during DNA replication origin licensing

Remus, D, Beuron, F, Tolun, G, Griffith, JD, Morris, EP, Diffley, JF

Cell 2009
23603117 An ORC/Cdc6/MCM2-7 complex is formed in a multistep reaction to serve as a platform for MCM double-hexamer assembly

Fernández-Cid, A, Riera, A, Tognetti, S, Herrera, MC, Samel, S, Evrin, C, Winkler, C, Gardenal, E, Uhle, S, Speck, C

Mol Cell 2013
23851460 Cryo-EM structure of a helicase loading intermediate containing ORC-Cdc6-Cdt1-MCM2-7 bound to DNA

Sun, J, Evrin, C, Samel, SA, Fernández-Cid, A, Riera, A, Kawakami, H, Stillman, B, Speck, C, Li, H

Nat Struct Mol Biol 2013
Participants
Input
Output
Orthologous Events
CDT1 binds MCM2-7 (Homo sapiens)
Authored
Kusic-Tisma, J  (2021-10-27)
Created
Orlic-Milacic, M  (2021-08-01)
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