Reactome | CDT1 binds MCM2-7

CDT1 binds MCM2-7

Stable Identifier

R-HSA-9749286

Type

Reaction [binding]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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While the interaction between CDT1 and the MCM2-7 complex was confirmed in human (Wu et al. 2014) and other mammalian cells (Cook et al. 2004), as well as in the fruit fly (Costa et al. 2011), the mechanism of interaction has only been studied in detail in yeast. From yeast studies, CDT1 binds to the MCM2-7 complex by interacting with its MCM2 and MCM6 subunits (Kawasaki et al. 2006; Remus et al. 2009), resulting in the formation of a hetero-heptameric CDT1:MCM2-7 complex (Sun et al. 2013). CDT1 binding relieves the autoinhibitory action of the MCM6 subunit that prevents association of the MCM2-7 complex with the complex of ORC and CDC6 in the absence of CDT1 (Fernández-Cid et al. 2013, Sun et al. 2013).

Literature References

PubMed ID	Title	Journal	Year
16824194	Reconstitution of Saccharomyces cerevisiae prereplicative complex assembly in vitro Seki, T, Kojima, A, Kawasaki, Y, Kim, HD, Sugino, A	Genes Cells	2006
19896182	Concerted loading of Mcm2-7 double hexamers around DNA during DNA replication origin licensing Beuron, F, Remus, D, Tolun, G, Morris, EP, Diffley, JF, Griffith, JD	Cell	2009
14672932	The regulated association of Cdt1 with minichromosome maintenance proteins and Cdc6 in mammalian cells Nevins, JR, Chasse, DA, Cook, JG	J Biol Chem	2004
25231993	Geminin inhibits a late step in the formation of human pre-replicative complexes Santos, RE, Wu, M, Lu, W, Frattini, MG, Kelly, TJ	J Biol Chem	2014
23603117	An ORC/Cdc6/MCM2-7 complex is formed in a multistep reaction to serve as a platform for MCM double-hexamer assembly Fernández-Cid, A, Speck, C, Samel, S, Gardenal, E, Winkler, C, Riera, A, Herrera, MC, Evrin, C, Uhle, S, Tognetti, S	Mol Cell	2013
23851460	Cryo-EM structure of a helicase loading intermediate containing ORC-Cdc6-Cdt1-MCM2-7 bound to DNA Fernández-Cid, A, Li, H, Samel, SA, Speck, C, Riera, A, Sun, J, Kawakami, H, Evrin, C, Stillman, B	Nat Struct Mol Biol	2013