CDT1 binds MCM2-7

Stable Identifier
Reaction [binding]
Homo sapiens
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While the interaction between CDT1 and the MCM2-7 complex was confirmed in human (Wu et al. 2014) and other mammalian cells (Cook et al. 2004), as well as in the fruit fly (Costa et al. 2011), the mechanism of interaction has only been studied in detail in yeast. From yeast studies, CDT1 binds to the MCM2-7 complex by interacting with its MCM2 and MCM6 subunits (Kawasaki et al. 2006; Remus et al. 2009), resulting in the formation of a hetero-heptameric CDT1:MCM2-7 complex (Sun et al. 2013). CDT1 binding relieves the autoinhibitory action of the MCM6 subunit that prevents association of the MCM2-7 complex with the complex of ORC and CDC6 in the absence of CDT1 (Fernández-Cid et al. 2013, Sun et al. 2013).
Literature References
PubMed ID Title Journal Year
16824194 Reconstitution of Saccharomyces cerevisiae prereplicative complex assembly in vitro

Seki, T, Kojima, A, Kawasaki, Y, Kim, HD, Sugino, A

Genes Cells 2006
19896182 Concerted loading of Mcm2-7 double hexamers around DNA during DNA replication origin licensing

Beuron, F, Remus, D, Tolun, G, Morris, EP, Diffley, JF, Griffith, JD

Cell 2009
14672932 The regulated association of Cdt1 with minichromosome maintenance proteins and Cdc6 in mammalian cells

Nevins, JR, Chasse, DA, Cook, JG

J Biol Chem 2004
25231993 Geminin inhibits a late step in the formation of human pre-replicative complexes

Santos, RE, Wu, M, Lu, W, Frattini, MG, Kelly, TJ

J Biol Chem 2014
23603117 An ORC/Cdc6/MCM2-7 complex is formed in a multistep reaction to serve as a platform for MCM double-hexamer assembly

Fernández-Cid, A, Speck, C, Samel, S, Gardenal, E, Winkler, C, Riera, A, Herrera, MC, Evrin, C, Uhle, S, Tognetti, S

Mol Cell 2013
23851460 Cryo-EM structure of a helicase loading intermediate containing ORC-Cdc6-Cdt1-MCM2-7 bound to DNA

Fernández-Cid, A, Li, H, Samel, SA, Speck, C, Riera, A, Sun, J, Kawakami, H, Evrin, C, Stillman, B

Nat Struct Mol Biol 2013
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