SBH1:GET3:ATP:GET4:MDY2:SGT2 dissociates and GET3 hydrolyzes ATP yielding SBH1:GET3:ADP

Stable Identifier
R-SCE-9629050
Type
Reaction [omitted]
Species
Saccharomyces cerevisiae
Compartment
General
SVG |   | PPTX  | SBGN
SBH1:GET3:ATP:GET4:MDY2:SGT2 dissociates and GET3 hydrolyzes ATP yielding SBH1:GET3:ADP

A tail-anchored protein such as SBH1 (Sec61beta) is transferred from SGT2 to a GET3:ATP dimer via the GET4:MDY2 (GET4:GET5) complex (Chartron et al. 2010, Gristick et al. 2014, Gristick et al. 2015, Mateja et al. 2015). After transfer the SBH1:GET3:ATP complex dissociates from GET4:MDY2 and ATP is hydrolyzed by the GET3 dimer (Gristick et al. 2014, Chio et al. 2017), resulting in a change in conformation and a change in affinity for other molecules in the insertion pathway (Rome et al. 2014, Chio et al. 2017). The timing of the hydrolysis of ATP is uncertain. GET4:MDY2 inhbits ATP hydrolysis by GET3 so ATP hydrolysis may occur after GET3:ATP has dissociated from GET4:MDY2 (Rome et al. 2013, Gristick et al. 2014).

Literature References
PubMed ID Title Journal Year
25368153 Differential gradients of interaction affinities drive efficient targeting and recycling in the GET pathway

Rome, ME, Chio, US, Rao, M, Gristick, H, Shan, SO

Proc. Natl. Acad. Sci. U.S.A. 2014
20554915 Structural characterization of the Get4/Get5 complex and its interaction with Get3

Chartron, JW, Suloway, CJ, Zaslaver, M, Clemons, WM

Proc. Natl. Acad. Sci. U.S.A. 2010
26451041 Mechanism of Assembly of a Substrate Transfer Complex during Tail-anchored Protein Targeting

Gristick, HB, Rome, ME, Chartron, JW, Rao, M, Hess, S, Shan, SO, Clemons, WM

J. Biol. Chem. 2015
24727835 Crystal structure of ATP-bound Get3-Get4-Get5 complex reveals regulation of Get3 by Get4

Gristick, HB, Rao, M, Chartron, JW, Rome, ME, Shan, SO, Clemons, WM

Nat. Struct. Mol. Biol. 2014
28973888 A protean clamp guides membrane targeting of tail-anchored proteins

Chio, US, Chung, S, Weiss, S, Shan, SO

Proc. Natl. Acad. Sci. U.S.A. 2017
25745174 Protein targeting. Structure of the Get3 targeting factor in complex with its membrane protein cargo

Mateja, A, Paduch, M, Chang, HY, Szydlowska, A, Kossiakoff, AA, Hegde, RS, Keenan, RJ

Science 2015
23610396 Precise timing of ATPase activation drives targeting of tail-anchored proteins

Rome, ME, Rao, M, Clemons, WM, Shan, SO

Proc. Natl. Acad. Sci. U.S.A. 2013
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