Intracellular proteins are targeted for proteolytic degradation in lysosome with the aid of chaperones. Heat shock cognate 71 kDa protein (Hspa8) transports substrates from the cytosol to the lysosomal membrane where it binds to Lysosome-associated membrane glycoprotein 2 (Lamp2). Subsequently, Lamp2 forms a multimeric complex and transfers the substrate into the lumen. The stability of this complex is regulated by the dynamics of Hspa8. Cytosolic Hspa8 binds with Lamp2 multimers in the lysosomal membrane and trigger their disassembly into monomeric units (Bandyopadhyay U et al. 2008). Hspa8 dissociates from Lamp2 to make it available for further substrate autophagy.