Complexed Piwil1 binds pre-piRNA

Stable Identifier
R-MMU-5615685
Type
Reaction [binding]
Species
Mus musculus
Compartment
cytosol
ReviewStatus
5/5
General
SVG | 
PNG
Low
Medium
High
 | PPTX  | SBGN
Complexed Piwil1 binds pre-piRNA
After cleavage by Pld6 at the 5' end, the pre-piRNA is bound by Piwil1 (also known as Miwi) (Grivna et al. 2006). Methylated arginine residues on Piwil1 (Vagin et al. 2009) interact with Tdrd6 (Vasileva et al. 2009) and Tdrkh (Chen et al. 2009, Saxe et al. 2013), both of which are required for piRNA biogenesis. Binding by Piwil1 is believed to be selective for pre-piRNAs that have uracil residues at their 5' ends.
Literature References
PubMed ID Title Journal Year
19584108 Proteomic analysis of murine Piwi proteins reveals a role for arginine methylation in specifying interaction with Tudor family members

Vagin, VV, Wohlschlegel, J, Qu, J, Jonsson, Z, Huang, X, Chuma, S, Girard, A, Sachidanandam, R, Hannon, GJ, Aravin, AA

Genes Dev. 2009
19918066 Mouse Piwi interactome identifies binding mechanism of Tdrkh Tudor domain to arginine methylated Miwi

Chen, C, Jin, J, James, DA, Adams-Cioaba, MA, Park, JG, Guo, Y, Tenaglia, E, Xu, C, Gish, G, Min, J, Pawson, T

Proc. Natl. Acad. Sci. U.S.A. 2009
19345099 Tdrd6 is required for spermiogenesis, chromatoid body architecture, and regulation of miRNA expression

Vasileva, A, Tiedau, D, Firooznia, A, Müller-Reichert, T, Jessberger, R

Curr. Biol. 2009
16766680 A novel class of small RNAs in mouse spermatogenic cells

Grivna, ST, Beyret, E, Wang, Z, Lin, H

Genes Dev. 2006
16938833 MIWI associates with translational machinery and PIWI-interacting RNAs (piRNAs) in regulating spermatogenesis

Grivna, ST, Pyhtila, B, Lin, H

Proc. Natl. Acad. Sci. U.S.A. 2006
23714778 Tdrkh is essential for spermatogenesis and participates in primary piRNA biogenesis in the germline

Saxe, JP, Chen, M, Zhao, H, Lin, H

EMBO J. 2013
Participants
Input
Output
Orthologous Events
Complexed PIWIL1 binds pre-piRNA (Homo sapiens)
Authored
May, B  (2014-07-29)
Reviewed
Saito, K  (2014-10-25)
Created
May, B  (2014-07-29)
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