Complexed Piwil2 binds pre-piRNA

Stable Identifier
Reaction [binding]
Mus musculus
SVG |   | PPTX  | SBGN
Complexed Piwil2 binds pre-piRNA

After cleavage by Pld6 at the 5' end, the pre-piRNA is bound by Piwil2 (also known as Mili) (Aravin et al. 2006). Piwil2 interacts with several other proteins, including Tdrd1 Aravin et al. 2009, Kojima et al. 2009, Reuter et al. 2009, Vagin et al. 2009, Mathioudakis et al. 2012), Tdrd12 (Pandey et al. 2013), Ddx4 (Mvh, Kuramochi-Miyagawa et al. 2004), Asz (Ma et al. 2009), and Mov10l (Frost et al. 2010, Zheng et al. 2010), all of which affect piRNA biogenesis. Tudor-containing proteins Tdrd1 and Tdrd12 bind Piwil2 via methylated arginine residues (Vagin et al. 2009). Binding by Piwil2 is believed to be selective for pre-piRNAs that have uracil residues at their 5' ends.

Literature References
PubMed ID Title Journal Year
24067652 Tudor domain containing 12 (TDRD12) is essential for secondary PIWI interacting RNA biogenesis in mice

Pandey, RR, Tokuzawa, Y, Yang, Z, Hayashi, E, Ichisaka, T, Kajita, S, Asano, Y, Kunieda, T, Sachidanandam, R, Chuma, S, Yamanaka, S, Pillai, RS

Proc. Natl. Acad. Sci. U.S.A. 2013
22996915 The multiple Tudor domain-containing protein TDRD1 is a molecular scaffold for mouse Piwi proteins and piRNA biogenesis factors

Mathioudakis, N, Palencia, A, Kadlec, J, Round, A, Tripsianes, K, Sattler, M, Pillai, RS, Cusack, S

RNA 2012
19584108 Proteomic analysis of murine Piwi proteins reveals a role for arginine methylation in specifying interaction with Tudor family members

Vagin, VV, Wohlschlegel, J, Qu, J, Jonsson, Z, Huang, X, Chuma, S, Girard, A, Sachidanandam, R, Hannon, GJ, Aravin, AA

Genes Dev. 2009
19465913 Loss of the Mili-interacting Tudor domain-containing protein-1 activates transposons and alters the Mili-associated small RNA profile

Reuter, M, Chuma, S, Tanaka, T, Franz, T, Stark, A, Pillai, RS

Nat. Struct. Mol. Biol. 2009
20547853 MOV10L1 is necessary for protection of spermatocytes against retrotransposons by Piwi-interacting RNAs

Frost, RJ, Hamra, FK, Richardson, JA, Qi, X, Bassel-Duby, R, Olson, EN

Proc. Natl. Acad. Sci. U.S.A. 2010
16751777 A novel class of small RNAs bind to MILI protein in mouse testes

Aravin, A, Gaidatzis, D, Pfeffer, S, Lagos-Quintana, M, Landgraf, P, Iovino, N, Morris, P, Brownstein, MJ, Kuramochi-Miyagawa, S, Nakano, T, Chien, M, Russo, JJ, Ju, J, Sheridan, R, Sander, C, Zavolan, M, Tuschl, T

Nature 2006
19730684 GASZ is essential for male meiosis and suppression of retrotransposon expression in the male germline

Ma, L, Buchold, GM, Greenbaum, MP, Roy, A, Burns, KH, Zhu, H, Han, DY, Harris, RA, Coarfa, C, Gunaratne, PH, Yan, W, Matzuk, MM

PLoS Genet. 2009
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Aravin, AA, van der Heijden, GW, CastaƱeda, J, Vagin, VV, Hannon, GJ, Bortvin, A

PLoS Genet. 2009
14736746 Mili, a mammalian member of piwi family gene, is essential for spermatogenesis

Kuramochi-Miyagawa, S, Kimura, T, Ijiri, TW, Isobe, T, Asada, N, Fujita, Y, Ikawa, M, Iwai, N, Okabe, M, Deng, W, Lin, H, Matsuda, Y, Nakano, T

Development 2004
20534472 Mouse MOV10L1 associates with Piwi proteins and is an essential component of the Piwi-interacting RNA (piRNA) pathway

Zheng, K, Xiol, J, Reuter, M, Eckardt, S, Leu, NA, McLaughlin, KJ, Stark, A, Sachidanandam, R, Pillai, RS, Wang, PJ

Proc. Natl. Acad. Sci. U.S.A. 2010
19735482 Associations between PIWI proteins and TDRD1/MTR-1 are critical for integrated subcellular localization in murine male germ cells

Kojima, K, Kuramochi-Miyagawa, S, Chuma, S, Tanaka, T, Nakatsuji, N, Kimura, T, Nakano, T

Genes Cells 2009
Orthologous Events