PORCN palmitoleoylates WNT3a

Stable Identifier
R-MMU-3238687
Type
Reaction [transition]
Species
Mus musculus
Compartment
endoplasmic reticulum lumen
ReviewStatus
5/5
General
SVG | 
PNG
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High
 | PPTX  | SBGN
PORCN palmitoleoylates WNT3a
Mouse WNT3a has been shown to be acylated at conserved S209 with the monounsaturated fatty acid palmitoleic acid (C16:1). Acylation is required for WNT3a secretion from the cell, as WNT3a (S209A) protein is retained in the ER. Acylation depends on the putative O-acyl-transferase PORCN, which was first identified as a Drosophila segment polarity gene that is required for the normal distribution of Wg in embryos (Kadowaka et al, 1996). In porcn-deficient cells, WNT3a accumulates in an unacylated form in the ER (Takada et al, 2006). PORCN-dependent acylation is required for the processing of other mouse WNT ligands (Tanaka, 2002).
Literature References
PubMed ID Title Journal Year
10866835 The evolutionarily conserved porcupine gene family is involved in the processing of the Wnt family

Tanaka, K, Okabayashi, K, Asashima, M, Perrimon, N, Kadowaki, T

Eur. J. Biochem. 2000
17141155 Monounsaturated fatty acid modification of Wnt protein: its role in Wnt secretion

Takada, R, Satomi, Y, Kurata, T, Ueno, N, Norioka, S, Kondoh, H, Takao, T, Takada, S

Dev. Cell 2006
8985181 The segment polarity gene porcupine encodes a putative multitransmembrane protein involved in Wingless processing

Kadowaki, T, Wilder, E, Klingensmith, J, Zachary, K, Perrimon, N

Genes Dev. 1996
Participants
Input
Output
Catalyst Activity

O-acyltransferase activity of PORCN [endoplasmic reticulum membrane]

Physical Entity
PORCN [endoplasmic reticulum membrane] (Mus musculus)
Activity
O-acyltransferase activity (GO:0008374)
Orthologous Events
PORCN palmitoleoylates N-glycosyl WNTs (Homo sapiens)
Authored
Rothfels, K  (2013-04-06)
Reviewed
Boutros, M  (2013-05-24)
Created
Rothfels, K  (2013-03-27)
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