PORCN palmitoleoylates WNT3a

Stable Identifier
Reaction [transition]
Mus musculus
SVG |   | PPTX  | SBGN
PORCN palmitoleoylates WNT3a

Mouse WNT3a has been shown to be acylated at conserved S209 with the monounsaturated fatty acid palmitoleic acid (C16:1). Acylation is required for WNT3a secretion from the cell, as WNT3a (S209A) protein is retained in the ER. Acylation depends on the putative O-acyl-transferase PORCN, which was first identified as a Drosophila segment polarity gene that is required for the normal distribution of Wg in embryos (Kadowaka et al, 1996). In porcn-deficient cells, WNT3a accumulates in an unacylated form in the ER (Takada et al, 2006). PORCN-dependent acylation is required for the processing of other mouse WNT ligands (Tanaka, 2002).

Literature References
PubMed ID Title Journal Year
17141155 Monounsaturated fatty acid modification of Wnt protein: its role in Wnt secretion

Takada, R, Satomi, Y, Kurata, T, Ueno, N, Norioka, S, Kondoh, H, Takao, T, Takada, S

Dev. Cell 2006
10866835 The evolutionarily conserved porcupine gene family is involved in the processing of the Wnt family

Tanaka, K, Okabayashi, K, Asashima, M, Perrimon, N, Kadowaki, T

Eur. J. Biochem. 2000
8985181 The segment polarity gene porcupine encodes a putative multitransmembrane protein involved in Wingless processing

Kadowaki, T, Wilder, E, Klingensmith, J, Zachary, K, Perrimon, N

Genes Dev. 1996
Catalyst Activity

O-acyltransferase activity of PORCN [endoplasmic reticulum membrane]

Orthologous Events
Cite Us!