CHD6 binds influenza polymerase and nucleoprotein

Stable Identifier
R-HSA-9944250
Type
Reaction [binding]
Species
Homo sapiens
Related Species
Influenza A virus
Compartment
nucleoplasm
ReviewStatus
3/5
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CHD6 binds influenza polymerase and nucleoprotein
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CHD6 interacts with all three components (PB1, PB2 and PA) of the influenza viral polymerase as well as with the nucleoprotein NP to negatively regulate viral replication and transcription in the nucleus (Huarte et al, 2001; Alfonso et al, 2011; Alfonso et al, 2013).
Negative strand viral RNA, -ssRNA, packaged into ribonucleoprotein particles with NP is the template for both the viral-polymerase-dependent mRNA synthesis and positive strand complementary RNA (cRNA) synthesis. cRNA is then itself a template for further rounds of synthesis of mRNA and vRNA (reviewed in Du et al, 2023; Eisfeld et al, 2015).
Various lines of evidence support an association between viral replication and transcription machinery and host chromatin (Bui et al, 2000; Bukrinskaya et al, 1979; Jackson et al, 1982; Lopez-Turiso et al, 1990; reviewed in Eisfeld et al, 2015; Du et al, 2023). Transcription of viral mRNA from the negative strand RNA viral template depends on priming by 10-12 base pair oligonucleotides scavenged from newly synthesized host mRNA molecules in a process that also depends on host splicing machinery (Plotch et al, 1982; Lamb et al, 1982). In vitro, NP is known to associate with host histone tails (Garcia-Robles et al, 2005). In separate studies, CHD6 was shown to co-localize with RNA polymerase II at sites of mRNA synthesis (Lutz et al, 2006). In studies from the Nieto lab, CHD6 was shown to negatively regulate influenza virus transcription and replication, but also to be a target of viral-mediated degradation along with RNA polymerase II (Huarte et al, 2001; Alfonso et al, 2011; Alfonso et al, 2013).
Literature References
PubMed ID Title Journal Year
10644350 Role of the influenza virus M1 protein in nuclear export of viral ribonucleoproteins

Bui, M, Wills, EG, Helenius, A, Whittaker, GR

J Virol 2000
2392881 The synthesis of influenza virus negative-strand RNA takes place in insoluble complexes present in the nuclear matrix fraction

López-Turiso, JA, Martínez, C, Tanaka, T, Ortín, J

Virus Res 1990
11507205 PA subunit from influenza virus polymerase complex interacts with a cellular protein with homology to a family of transcriptional activators

Huarte, M, Sanz-Ezquerro, JJ, Roncal, F, Ortín, J, Nieto, A

J Virol 2001
541671 Cytoplasmic and nuclear input virus RNPs in influenza virus-infected cells

Bukrinskaya, AG, Vorkunova, GK, Vorkunova, NK

J Gen Virol 1979
6184879 Spliced and unspliced messenger RNAs synthesized from cloned influenza virus M DNA in an SV40 vector: expression of the influenza virus membrane protein (M1)

Lamb, RA, Lai, CJ

Virology 1982
7063035 Influenza virus RNA is synthesized at fixed sites in the nucleus

Jackson, DA, Caton, AJ, McCready, SJ, Cook, PR

Nature 1982
6261960 A unique cap(m7GpppXm)-dependent influenza virion endonuclease cleaves capped RNAs to generate the primers that initiate viral RNA transcription

Plotch, SJ, Bouloy, M, Ulmanen, I, Krug, RM

Cell 1981
23408615 CHD6, a cellular repressor of influenza virus replication, is degraded in human alveolar epithelial cells and mice lungs during infection

Alfonso, R, Rodriguez, A, Rodriguez, P, Lutz, T, Nieto, A

J Virol 2013
36309307 Revisiting influenza A virus life cycle from a perspective of genome balance

Du, R, Cui, Q, Chen, Z, Zhao, X, Lin, X, Rong, L

Virol Sin 2023
25417656 At the centre: influenza A virus ribonucleoproteins

Eisfeld, AJ, Neumann, G, Kawaoka, Y

Nat Rev Microbiol 2015
15661164 Interaction of influenza virus proteins with nucleosomes

Garcia-Robles, I, Akarsu, H, Muller, CW, Ruigrok, RW, Baudin, F

Virology 2005
21899694 CHD6 chromatin remodeler is a negative modulator of influenza virus replication that relocates to inactive chromatin upon infection

Alfonso, R, Lutz, T, Rodriguez, A, Chavez, JP, Rodriguez, P, Gutierrez, S, Nieto, A

Cell Microbiol 2011
17027977 CHD6 is a DNA-dependent ATPase and localizes at nuclear sites of mRNA synthesis

Lutz, T, Stöger, R, Nieto, A

FEBS Lett 2006
Participants
Input
Output
Participates
as an event of
Disease
Name Identifier Synonyms
influenza DOID:8469 Influenza with other manifestations (disorder), Influenza with other manifestations NOS (disorder), flu, Influenza with other manifestations, Influenza with non-respiratory manifestation (disorder), influenza with non-respiratory manifestation
Cross References
Mondo
Authored
Rothfels, K  (2025-02-07)
Created
Rothfels, K  (2025-04-03)
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