ANK3 (Ankyrin-G), a scaffold protein that links membrane proteins to the spectrin-based cytoskeleton, binds to the complex of CDH1 (E-cadherin) and CTNNB1 (beta-catenin) through the interaction between the cytosolic tail of CDH1 and ankyrin repeats of ANK3 (Kizhatil et al. 2007: endogenous proteins in human bronchoepithelial cells and early mouse embryos and recombinant human CDH1, rat ANK3 and CTNNB1 of unspecified origin; Kong et al. 2023: study performed in canine MDCK cell line). It is assumed that ANK3 can similarly bind to the complex of CDH1 and JUP (plakoglobin). A recombinant human CDH1 deficient in ANK3 binding exhibits defective accumulation at the lateral plasma membrane when expressed in MDCK cells (Jenkins et al. 2013; Kong et al. 2023). ANK3 and beta-2-spectrin were shown to be required for accumulation of CDH1 at the lateral membrane in both human bronchial epithelial (HBE) cells, human HEK293 cells, and early mouse embryos (Kizhatil et al. 2007). Recombinant human CDH1 deficient for ANK3 binding is retained in Golgi when expressed in HBE cells (Kizhatil et al. 2007). ANK3 likely couples CDH1 to spectrin, which then facilitates transport of CDH1 to the plasma membrane via microtubule-dependent post-Golgi carriers (Kizhatil et al. 2007).