Immature virion localizes to exocytic vesicle

Stable Identifier
R-HSA-9923837
Type
Reaction [uncertain]
Species
Homo sapiens
Related Species
Dengue virus type 2
Compartment
Golgi lumen, exocytic vesicle
ReviewStatus
5/5
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Immature virion localizes to exocytic vesicle
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The DENV immature virion localizes to exocytic vesicles via the secretory pathway (Martins et al., 2018; reviewed by Apte-Sengupta et al., 2014). A requirement for the transport is Clathrin interactor 1 (CLINT1), phosphorylated at Thr-294 by the BMP-2-inducible protein kinase (BMP2K, BIKE) (Schor et al., 2022). It has also been shown that DENV uses the secretory autophagosome pathway for egress and that this requires the Tyrosine-protein kinase LYN (Li et al., 2020). DENV also exploits autophagy vesicles for transmission by human dendritic cells (Cloherty et al., 2024; for a review on viruses and secretory autophagy see Pleet et al., 2018)

Dasatinib, a small molecule approved for the treatment of leukemia, inhibits tyrosine kinases like LYN and affects DENV virion assembly (Chu & Yang, 2007; Wispelaere et al., 2013; reviewed in García-Cárceles et al., 2022).
Literature References
PubMed ID Title Journal Year
17360676 c-Src protein kinase inhibitors block assembly and maturation of dengue virus

Chu, JJ, Yang, PL

Proc Natl Acad Sci U S A 2007
33060596 Lyn kinase regulates egress of flaviviruses in autophagosome-derived organelles

Li, MY, Naik, TS, Siu, LYL, Acuto, O, Spooner, E, Wang, P, Yang, X, Lin, Y, Bruzzone, R, Ashour, J, Evans, MJ, Sanyal, S

Nat Commun 2020
35452674 The cargo adapter protein CLINT1 is phosphorylated by the Numb-associated kinase BIKE and mediates dengue virus infection

Schor, S, Pu, S, Nicolaescu, V, Azari, S, Kõivomägi, M, Karim, M, Cassonnet, P, Saul, S, Neveu, G, Yueh, A, Demeret, C, Skotheim, JM, Jacob, Y, Randall, G, Einav, S

J Biol Chem 2022
25462445 Coupling of replication and assembly in flaviviruses

Apte-Sengupta, S, Sirohi, D, Kuhn, RJ

Curr Opin Virol 2014
30406039 Autophagy, EVs, and Infections: A Perfect Question for a Perfect Time

Pleet, ML, Branscome, H, DeMarino, C, Pinto, DO, Zadeh, MA, Rodriguez, M, Sariyer, IK, El-Hage, N, Kashanchi, F

Front Cell Infect Microbiol 2018
23616652 The small molecules AZD0530 and dasatinib inhibit dengue virus RNA replication via Fyn kinase

de Wispelaere, M, LaCroix, AJ, Yang, PL

J Virol 2013
30131785 Characterization of Dendritic Cell-Derived Extracellular Vesicles During Dengue Virus Infection

Martins, ST, Kuczera, D, Lötvall, J, Bordignon, J, Alves, LR

Front Microbiol 2018
38863700 Dengue virus exploits autophagy vesicles and secretory pathways to promote transmission by human dendritic cells

Cloherty, APM, Rader, AG, Patel, KS, Eisden, TTHD, van Piggelen, S, Schreurs, RRCE, Ribeiro, CMS

Front Immunol 2024
33970631 Kinase Inhibitors as Underexplored Antiviral Agents

García-Cárceles, J, Caballero, E, Gil, C, Martínez, A

J Med Chem 2022
Participants
Input
Output
Participates
as an event of
Event Information
Go Biological Process
intracellular transport of virus (0075733)
This event is regulated
Negatively by
Regulator
dasatinib [cytosol]
Requirements
Disease
Name Identifier Synonyms
dengue disease DOID:12205 breakbone fever, Dengue Fever, classic dengue
Cross References
Mondo
Authored
Stephan, R  (2024-10-03)
Reviewed
Mansur, DS  (2025-10-29)
Roque Ascenção, F  (2025-10-29)
Created
Stephan, R  (2024-10-03)
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