DENV virion buds into ER lumen

Stable Identifier
R-HSA-9923836
Type
Reaction [uncertain]
Species
Homo sapiens
Related Species
Dengue virus type 2
Compartment
endoplasmic reticulum lumen, endoplasmic reticulum membrane
ReviewStatus
5/5
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DENV virion buds into ER lumen
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The nucleocapsid complex, localized on the cytosolic side of the ER membrane, associates with the viral glycoproteins on the lumenal side of the ER membrane, and buds into the ER lumen, forming an immature virion (reviewed by Apte-Sengupta et al., 2014; Barnard et al., 2021). Budding can also happen without capsids (e.g., formation of subviral particles), indicating that the mechanisms ensuring efficient nucleocapsid incorporation into budding viral membranes are not well understood (reviewed by Apte-Sengupta et al., 2014). The lipid content of DENV-2 virions differs significantly when produced in mammalian (rhesus monkey kidney LLC-MK2) versus insect (Aedes albopictus C6/36) cells (Hitakarun et al., 2022). As placeholder for all included lipids we show phosphatidylserine (PS). Formation of infectious DENV-2 virions and their subsequent secretion depends on Y-box-binding protein 1 (YBX1) (Diosa-Toro et al., 2022, using DENV-2 NGC), as well as nucleolin (NCL; Balinsky et al., 2013, using DENV-2 Tonga/74; reviewed in Barnard et al., 2021). Tumor susceptibility gene 101 (TSG101) protein, part of the Endosomal Sorting Complexes required for Transport (ESCRT), is required for viral budding (Tabata et al., 2016, using DENV-2 NGC).
Literature References
PubMed ID Title Journal Year
30853381 Stearoyl-CoA desaturase-1 is required for flavivirus RNA replication

Hishiki, T, Kato, F, Nio, Y, Watanabe, S, Wen Tan, NW, Yamane, D, Miyazaki, Y, Lin, CC, Suzuki, R, Tajima, S, Lim, CK, Saijo, M, Hijikata, M, Vasudevan, SG, Takasaki, T

Antiviral Res 2019
23241927 Cryo-EM structure of the mature dengue virus at 3.5-Å resolution

Zhang, X, Ge, P, Yu, X, Brannan, JM, Bi, G, Zhang, Q, Schein, S, Zhou, ZH

Nat Struct Mol Biol 2013
25462445 Coupling of replication and assembly in flaviviruses

Apte-Sengupta, S, Sirohi, D, Kuhn, RJ

Curr Opin Virol 2014
30118512 Stearoly-CoA desaturase 1 differentiates early and advanced dengue virus infections and determines virus particle infectivity

Gullberg, RC, Steel, JJ, Pujari, V, Rovnak, J, Crick, DC, Perera, R

PLoS Pathog 2018
35189699 Y-Box Binding Protein 1 Interacts with Dengue Virus Nucleocapsid and Mediates Viral Assembly

Diosa-Toro, M, Kennedy, DR, Chuo, V, Popov, VL, Pompon, J, Garcia-Blanco, MA

mBio 2022
27545892 Unique Requirement for ESCRT Factors in Flavivirus Particle Formation on the Endoplasmic Reticulum

Tabata, K, Arimoto, M, Arakawa, M, Nara, A, Saito, K, Omori, H, Arai, A, Ishikawa, T, Konishi, E, Suzuki, R, Matsuura, Y, Morita, E

Cell Rep 2016
39375358 Glycerophospholipid remodeling is critical for orthoflavivirus infection

Hehner, J, Schneider, L, Woitalla, A, Ott, B, Vu, KCT, Schöbel, A, Hain, T, Schwudke, D, Herker, E

Nat Commun 2024
36423175 Cell Type Variability in the Incorporation of Lipids in the Dengue Virus Virion

Hitakarun, A, Williamson, MK, Yimpring, N, Sornjai, W, Wikan, N, Arthur, CJ, Pompon, J, Davidson, AD, Smith, DR

Viruses 2022
24027323 Nucleolin interacts with the dengue virus capsid protein and plays a role in formation of infectious virus particles

Balinsky, CA, Schmeisser, H, Ganesan, S, Singh, K, Pierson, TC, Zoon, KC

J Virol 2013
33423940 Molecular Determinants of Flavivirus Virion Assembly

Barnard, TR, Abram, QH, Lin, QF, Wang, AB, Sagan, SM

Trends Biochem Sci 2021
Participants
Input
Output
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Event Information
Go Biological Process
viral budding from endoplasmic reticulum membrane (0046762)
This event is regulated
Requirements
Disease
Name Identifier Synonyms
dengue disease DOID:12205 breakbone fever, Dengue Fever, classic dengue
Cross References
Mondo
Authored
Stephan, R  (2024-10-03)
Reviewed
Mansur, DS  (2025-10-29)
Roque Ascenção, F  (2025-10-29)
Created
Stephan, R  (2024-10-03)
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