NS2B binds NS3

Stable Identifier
R-HSA-9918807
Type
Reaction [binding]
Species
Homo sapiens
Related Species
Dengue virus type 2
Compartment
cytosol
ReviewStatus
5/5
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NS2B binds NS3
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Dengue virus proteins NS2B and NS3 bind non-covalently. The NS2B:NS3 protease complex (aka NS2B3) is essential in both the viral multiplication cycle and is implicated in disease pathogenesis (Falgout et al., 1991; Noble et al., 2012; reviewed in Nitsche et al., 2014; Leonel et al., 2018). While anchored to membranes, the catalytic domain of the NS2B3 complex, including the hydrophilic region of NS2B that acts as a cofactor, is oriented towards the cytosol where it encounters and processes its substrates (see, e.g., Wahaab et al., 2021). DENV-1 NS2B3, depending on the state of replication, can localize to the nucleus, however (Gandhi et al., 2023).
Literature References
PubMed ID Title Journal Year
29147793 Pharmacophoric characteristics of dengue virus NS2B/NS3pro inhibitors: a systematic review of the most promising compounds

Leonel, CA, Lima, WG, Dos Santos, M, Ferraz, AC, Taranto, AG, de Magalhães, JC, Dos Santos, LL, Ferreira, JMS

Arch Virol 2018
35062249 Potential Role of Flavivirus NS2B-NS3 Proteases in Viral Pathogenesis and Anti-flavivirus Drug Discovery Employing Animal Cells and Models: A Review

Wahaab, A, Mustafa, BE, Hameed, M, Stevenson, NJ, Anwar, MN, Liu, K, Wei, J, Qiu, Y, Ma, Z

Viruses 2021
22031935 Ligand-bound structures of the dengue virus protease reveal the active conformation

Noble, CG, Seh, CC, Chao, AT, Shi, PY

J Virol 2012
25268322 Biochemistry and medicinal chemistry of the dengue virus protease

Nitsche, C, Holloway, S, Schirmeister, T, Klein, CD

Chem Rev 2014
2016768 Both nonstructural proteins NS2B and NS3 are required for the proteolytic processing of dengue virus nonstructural proteins

Falgout, B, Pethel, M, Zhang, YM, Lai, CJ

J Virol 1991
37534186 Differential localization of dengue virus protease affects cell homeostasis and triggers to thrombocytopenia

Gandhi, L, Maisnam, D, Rathore, D, Chauhan, P, Bonagiri, A, Venkataramana, M

iScience 2023
Participants
Input
Output
Participates
as an event of
Disease
Name Identifier Synonyms
dengue disease DOID:12205 breakbone fever, Dengue Fever, classic dengue
Cross References
Mondo
Authored
Stephan, R  (2024-08-18)
Reviewed
Mansur, DS  (2025-09-18)
Roque Ascenção, F  (2025-10-29)
Created
Stephan, R  (2024-08-18)
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