Reactome | E and N are recruited to the M lattice

E and N are recruited to the M lattice

Stable Identifier

R-HSA-9694444

Type

Reaction [binding]

Species

Homo sapiens

Related Species

Severe acute respiratory syndrome coronavirus 2

Compartment

endoplasmic reticulum-Golgi intermediate compartment membrane

ReviewStatus

5/5

Locations in the PathwayBrowser

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Disease (Homo sapiens)

- Infectious disease (Homo sapiens)
  - Viral Infection Pathways (Homo sapiens)
    - SARS-CoV Infections (Homo sapiens)
      - SARS-CoV-2 Infection (Homo sapiens)
        
        Late SARS-CoV-2 Infection Events (Homo sapiens)
        
        Virion Assembly and Release (Homo sapiens)
        
        E and N are recruited to the M lattice (Homo sapiens)

General

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This COVID-19 event has been created by a combination of computational inference (see https://reactome.org/documentation/inferred-events) from SARS-CoV-1 data and manual curation, as described in the summation for the overall SARS-CoV-2 infection pathway.

The ribonucleoprotein complex is recruited to the assembling virion through interactions with the M C-terminal tail, and appears to be independent of viral RNA (Hsieh et al, 2008; Hatakeyama et al, 2008; He et al, 2004; Luo et al, 2006; reviewed in Ujike and Taguchi, 2015). How much of this is fully conserved in SARS-COV-2 remains to be experimentally verified.

Literature References

PubMed ID	Title	Journal	Year
16254320	Assembly of severe acute respiratory syndrome coronavirus RNA packaging signal into virus-like particles is nucleocapsid dependent Hsieh, PK, Chang, CK, Lee, TT, Hsiao, CW, Huang, CC, Chen, IY, Kou, YH, Chang, MF, Huang, TH, Chang, SC	J. Virol.	2005
15507643	Generation of synthetic severe acute respiratory syndrome coronavirus pseudoparticles: implications for assembly and vaccine production Kong, WP, Huang, Y, Nabel, GJ, Yang, ZY	J. Virol.	2004
17530462	Coronavirus envelope protein: a small membrane protein with multiple functions Liao, Y, Liu, DX, Yuan, Q	Cell. Mol. Life Sci.	2007
15147946	Assembly of human severe acute respiratory syndrome coronavirus-like particles Lee, SP, Chao, YC, Ho, Y, Lin, PH, Liu, CY	Biochem. Biophys. Res. Commun.	2004
31133031	Coronavirus envelope protein: current knowledge Schoeman, D, Fielding, BC	Virol. J.	2019
25855243	Incorporation of spike and membrane glycoproteins into coronavirus virions Ujike, M, Taguchi, F	Viruses	2015
15351485	Characterization of protein-protein interactions between the nucleocapsid protein and membrane protein of the SARS coronavirus Li, Y, Tyler, S, Strocher, U, Booth, TF, Theriault, S, Bastien, N, Baker, L, He, R, Plummer, FA, Cao, J, Feldmann, H, Cutts, T, Ballantine, M, Li, X, Dobie, F, Leeson, A, Andonov, A	Virus Res.	2004
16684538	Biochemical evidence for the presence of mixed membrane topologies of the severe acute respiratory syndrome coronavirus envelope protein expressed in mammalian cells Liu, DX, Liao, Y, Tam, JP, Yuan, Q, Torres, J	FEBS Lett.	2006
16507314	Biochemical and functional characterization of the membrane association and membrane permeabilizing activity of the severe acute respiratory syndrome coronavirus envelope protein Liu, DX, Liao, Y, Tam, JP, Yuan, Q, Torres, J	Virology	2006
15474033	Efficient assembly and release of SARS coronavirus-like particles by a heterologous expression system Mortola, E, Roy, P	FEBS Lett.	2004
16343974	Severe acute respiratory syndrome coronavirus membrane protein interacts with nucleocapsid protein mostly through their carboxyl termini by electrostatic attraction Shen, X, Shen, C, Luo, H, Jiang, H, Wu, D, Chen, K	Int. J. Biochem. Cell Biol.	2006
15713601	The transmembrane oligomers of coronavirus protein E Parthasarathy, K, Liu, DX, Wang, J, Torres, J	Biophys. J.	2005
18703211	Dissection and identification of regions required to form pseudoparticles by the interaction between the nucleocapsid (N) and membrane (M) proteins of SARS coronavirus Kanai, T, Itoh, K, Shichijo, S, Miyoshi-Akiyama, T, Sasazuki, T, Kirikae, T, Fukushi, M, Hatakeyama, S, Komatsu, N, Ishida, I, Matsuoka, Y, Ueshiba, H	Virology	2008
18753196	The M, E, and N structural proteins of the severe acute respiratory syndrome coronavirus are required for efficient assembly, trafficking, and release of virus-like particles Nicholls, JM, Nal, B, Altmeyer, R, Bruzzone, R, Teoh, KT, Kien, F, Chan, CM, Escriou, N, Tsao, SW, Peiris, JS, Siu, YL, Lo, J	J. Virol.	2008
18792806	Interactions between M protein and other structural proteins of severe, acute respiratory syndrome-associated coronavirus Chen, SC, Li, HC, Lo, SY, Hsieh, YC	J. Biomed. Sci.	2008
16877062	The molecular biology of coronaviruses Masters, PS	Adv. Virus Res.	2006
31226023	Human Coronavirus: Host-Pathogen Interaction Fung, TS, Liu, DX	Annu. Rev. Microbiol.	2019
19322648	Expression and membrane integration of SARS-CoV E protein and its interaction with M protein Chen, SC, Li, HC, Ma, HC, Lo, SY	Virus Genes	2009

Participants

Input

Output

M lattice:E protein:encapsidated SARS-CoV-2 genomic RNA [endoplasmic reticulum-Golgi intermediate compartment membrane] (Severe acute respiratory syndrome coronavirus 2)

Participates

as an event of

Virion Assembly and Release (Homo sapiens)

Inferred From

E and N are recruited to the M lattice (Homo sapiens)

Disease

Name	Identifier	Synonyms
COVID-19	DOID:0080600	2019 Novel Coronavirus (2019-nCoV), Wuhan seafood market pneumonia virus infection, 2019-nCoV infection, Wuhan coronavirus infection

Authored

Rothfels, K (2020-07-30)

Reviewed

Acencio, ML (2020-09-09)

Created

Cook, J (2020-07-07)