Reactome | N-glycan trimming and elongation in the cis-Golgi

N-glycan trimming and elongation in the cis-Golgi

Stable Identifier

R-HSA-964739

Type

Pathway

Species

Homo sapiens

ReviewStatus

5/5

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N-glycan trimming and elongation in the cis-Golgi

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After the transport of the glycoprotein to the cis-Golgi, the pathway of N-glycosylation bifurcates. Some N-glycans can be moved to subsequent steps of the secretory pathway without further modifications, or alternatively, with the removal of a few mannoses (Oligo Mannoses pathway). In yeast and other unicellular species, a series of mannose residues are added (High Mannoses pathway). The presence of this modification is a major obstacle to the production of pharmaceutical drugs in yeast, where the HighMannose pathway must be inhibited or modified in order to avoid the presence of high mannose xenoglycans.
The first N-glycan modification step is the trimming of up to four mannoses by one of three mannosidase enzymes. Moreover, Glycoproteins that have not entered in the Calnexin/Calreticulin cycle or that have not had their glucose residues trimmed earlier in the ER, can enter the main pathway here due to the existence to an alternative route catalyzed by the enzyme Endomannosidase I (Schachter, 2000; Stanley et al, 2009)

Literature References

PubMed ID	Title	Journal	Year
	N-Glycans Stanley, P, Stanley, P, Schachter, H, Taniguchi, N, Varki, A, Cummings, RD, Esko, JD, Freeze, HH, Bertozzi, CR, Hart, GW, Etzler, ME		2009
11421343	The joys of HexNAc. The synthesis and function of N- and O-glycan branches Schachter, H	Glycoconj J	2000